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Decreased Serum microRNA-21, microRNA-25, microRNA-146a, and microRNA-181a in Autoimmune Diabetes: Potential Biomarkers for Diagnosis and Possible Involvement in Pathogenesis
Joint Authors
Ping, Fan
Li, Yu-xiu
Li, Wei
Liu, Yiwen
Ma, Minglei
Yu, Jie
Zhang, Huabing
Xu, Lingling
Source
International Journal of Endocrinology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
Previous studies have revealed dysregulated circulating microRNAs (miRNAs) in patients with type 1 diabetes (T1D).
Here, we explored the serum levels of miR-21, miR-25, miR-146a, and miR-181a in patients with autoimmune diabetes (T1D and latent autoimmune diabetes of adults (LADA)) compared with type 2 diabetes (T2D) and nondiabetic individuals.
Design, patients, and measurements.
The serum levels of miR-21, miR-25, miR-146a, and miR-181a in patients with T1D (n = 29), LADA (n = 16), and T2D (n = 31) and in nondiabetic individuals (n = 19) were determined by quantitative real-time polymerase chain reaction, and receiver-operating characteristic (ROC) curves were evaluated to determine the discriminatory performances of these four miRNAs.
Furthermore, target genes and pathways potentially modulated by these four miRNAs were predicted by bioinformatics analysis to investigate the possible functions of these miRNAs in autoimmune diabetes.
Subsequently, multiple logistic regression analysis was performed to identify independent predictors for autoimmune diabetes, and a nomogram was established.
Results.
miR-21, miR-25, miR-146a, and miR-181a were significantly downregulated in the serum of patients with autoimmune diabetes compared with those in T2D patients and nondiabetic individuals (p<0.001).
The areas under the ROC curves of these four miRNAs were greater than 0.80 (p<0.001).
Bioinformatics analysis suggested that miR-21, miR-25, miR-146a, and miR-181a regulated multiple genes in pathways associated with immunity, inflammatory responses, hyperglycemia, and metabolism, which are involved in the pathogenesis of autoimmune diabetes.
Multiple logistic regression analysis identified miR-25 (odds ratio (OR): 0.001, p<0.05), miR-146a (OR: 0.136, p<0.05), and fasting C-peptide levels (OR: 0.064, p<0.05) as independent predictors of autoimmune diabetes.
Conclusions.
miR-25 and miR-146a may serve as potential circulating biomarkers and provide insights into the pathogenesis of autoimmune diabetes.
American Psychological Association (APA)
Liu, Yiwen& Ma, Minglei& Yu, Jie& Ping, Fan& Zhang, Huabing& Li, Wei…[et al.]. 2019. Decreased Serum microRNA-21, microRNA-25, microRNA-146a, and microRNA-181a in Autoimmune Diabetes: Potential Biomarkers for Diagnosis and Possible Involvement in Pathogenesis. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1160082
Modern Language Association (MLA)
Liu, Yiwen…[et al.]. Decreased Serum microRNA-21, microRNA-25, microRNA-146a, and microRNA-181a in Autoimmune Diabetes: Potential Biomarkers for Diagnosis and Possible Involvement in Pathogenesis. International Journal of Endocrinology No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1160082
American Medical Association (AMA)
Liu, Yiwen& Ma, Minglei& Yu, Jie& Ping, Fan& Zhang, Huabing& Li, Wei…[et al.]. Decreased Serum microRNA-21, microRNA-25, microRNA-146a, and microRNA-181a in Autoimmune Diabetes: Potential Biomarkers for Diagnosis and Possible Involvement in Pathogenesis. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1160082
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1160082