Influence of Vanadium 4+ and 5+ Ions on the Differentiation and Activation of Human Osteoclasts
Joint Authors
Cadosch, Dieter
König, Matthias A.
Gautschi, Oliver P.
Filgueira, Luis
Simmen, Hans-Peter
Source
International Journal of Biomaterials
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-29
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
In the pathophysiology of implant failure, metal ions and inflammation-driven osteoclasts (OC) play a crucial role.
The aim of this study was to investigate whether vanadium (V) ions induce differentiation of monocytic OC precursors into osteoresorptive multinucleated cells.
In addition, the influence of V ions on the activation and function of in vitro generated OC was observed.
Methods.
Human monocytes and osteoclasts were isolated from peripheral blood monocytic cells (PBMCs).
Exposition with increasing concentrations (0–3 μM) of V4+/V5+ ions for 7 days followed.
Assessment of OC differentiation, cell viability, and resorptional ability was performed by standard colorimetric cell viability assay 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenil)-2H-tetrazolium (MTS), tartrate-resistant acid phosphatase (TRAP) expression, and functional resorption assays on bone slides during a period of 21 days.
Results.
No significant differences were noted between V4+/V5+ ions (p>0.05).
MTS showed significant reduction in cellular viability by V concentrations above 3 μM (p<0.05).
V concentrations above 0.5 μM showed negative effects on OC activation/differentiation.
Higher V concentrations showed negative effects on resorptive function (all p<0.05) without affecting cell viability.
V4+/V5+ concentrations below 3 μM have negative effects on OC differentiation/function without affecting cell survival.
Conclusion.
Vanadium-containing implants may reduce implant failure rate by influencing osteoclast activity at the bone-implant interface.
V-ligand complexes might offer new treatment options by accumulating in the bone.
American Psychological Association (APA)
König, Matthias A.& Gautschi, Oliver P.& Simmen, Hans-Peter& Filgueira, Luis& Cadosch, Dieter. 2017. Influence of Vanadium 4+ and 5+ Ions on the Differentiation and Activation of Human Osteoclasts. International Journal of Biomaterials،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1160097
Modern Language Association (MLA)
König, Matthias A.…[et al.]. Influence of Vanadium 4+ and 5+ Ions on the Differentiation and Activation of Human Osteoclasts. International Journal of Biomaterials No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1160097
American Medical Association (AMA)
König, Matthias A.& Gautschi, Oliver P.& Simmen, Hans-Peter& Filgueira, Luis& Cadosch, Dieter. Influence of Vanadium 4+ and 5+ Ions on the Differentiation and Activation of Human Osteoclasts. International Journal of Biomaterials. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1160097
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1160097