GNPDA2 Gene Affects Adipogenesis and Alters the Transcriptome Profile of Human Adipose-Derived Mesenchymal Stem Cells
Joint Authors
Zhao, Xiaoyuan
Wu, Lijun
Ma, Feifei
Zhang, Mei-Xian
Wu, Jianxin
Mi, Jie
Source
International Journal of Endocrinology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Genome-wide association studies have found an obesity-related single-nucleotide polymorphism rs10938397 near the glucosamine-6-phosphate deaminase 2 gene (GNPDA2) encoding, an enzyme that catalyzes the deamination of the glucosamine-6-phosphate involved in the hexosamine signaling pathway, but the molecular mechanisms underlying the missing link between GNPDA2 and obesity remain elusive.
Methods.
As obesity is accompanied by an increase in the size and the number of adipocytes, the present study investigates the possible mechanism of the GNPDA2 in adipogenesis using GeneChip® Human Transcriptome Array 2.0 in human adipose-derived mesenchymal stem cells.
Results.
We found that overexpression of GNPDA2 enhanced accumulation of lipid droplets, and knocking down the gene decreased accumulation of lipid droplets.
GO term enrichment analysis indicated that most differentially expressed genes (DEGs) affected by deficiency of GNPDA2 have functions to lipid and glucose metabolism.
Further KEGG enrichment analysis showed that the greatest proportion of DEGs are involved in thermogenesis, peroxisome proliferator-activated receptor (PPAR) signaling pathway, carbon metabolism, and fatty acid metabolism including fatty acid degradation, elongation, and biosynthesis.
Conclusion.
These findings suggest that GNPDA2 may be a critical gene for lipid and glucose metabolism, and the expression level of GNPDA2 alters the transcriptome profile of human adipose-derived mesenchymal stem cells.
American Psychological Association (APA)
Wu, Lijun& Ma, Feifei& Zhao, Xiaoyuan& Zhang, Mei-Xian& Wu, Jianxin& Mi, Jie. 2019. GNPDA2 Gene Affects Adipogenesis and Alters the Transcriptome Profile of Human Adipose-Derived Mesenchymal Stem Cells. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1165111
Modern Language Association (MLA)
Wu, Lijun…[et al.]. GNPDA2 Gene Affects Adipogenesis and Alters the Transcriptome Profile of Human Adipose-Derived Mesenchymal Stem Cells. International Journal of Endocrinology No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1165111
American Medical Association (AMA)
Wu, Lijun& Ma, Feifei& Zhao, Xiaoyuan& Zhang, Mei-Xian& Wu, Jianxin& Mi, Jie. GNPDA2 Gene Affects Adipogenesis and Alters the Transcriptome Profile of Human Adipose-Derived Mesenchymal Stem Cells. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1165111
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1165111