Allelic Switching of DLX5, GRB10, and SVOPL during Colorectal Cancer Tumorigenesis

Joint Authors

van Wezel, T.
Boot, Arnoud
Oosting, Jan
Doorn, Saskia
Ouahoud, Sarah
Ventayol Garcia, Marina
Ruano, Dina
Morreau, Hans

Source

International Journal of Genomics

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-04-10

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Biology

Abstract EN

Allele-specific expression (ASE) is found in approximately 20-30% of human genes.

During tumorigenesis, ASE changes due to somatic alterations that change the regulatory landscape.

In colorectal cancer (CRC), many chromosomes show frequent gains or losses while homozygosity of chromosome 7 is rare.

We hypothesized that genes essential to survival show allele-specific expression (ASE) on both alleles of chromosome 7.

Using a panel of 21 recently established low-passage CRC cell lines, we performed ASE analysis by hybridizing DNA and cDNA to Infinium HumanExome-12 v1 BeadChips containing cSNPs in 392 chromosome 7 genes.

The results of this initial analysis were extended and validated in a set of 89 paired normal mucosa and CRC samples.

We found that 14% of genes showed ASE in one or more cell lines and identified allelic switching of the potential cell survival genes DLX5, GRB10, and SVOPL on chromosome 7, whereby the most abundantly expressed allele in the normal tissue is the lowest expressed allele in the tumor and vice versa.

We established that this allelic switch does not result from loss of imprinting.

The allelic switching of SVOPL may be a result of transcriptional downregulation, while the exact mechanisms resulting in the allelic switching of DLX5 and GRB10 remain to be elucidated.

In conclusion, our results show that profound changes take place in allelic transcriptional regulation during the tumorigenesis of CRC.

American Psychological Association (APA)

Boot, Arnoud& Oosting, Jan& Doorn, Saskia& Ouahoud, Sarah& Ventayol Garcia, Marina& Ruano, Dina…[et al.]. 2019. Allelic Switching of DLX5, GRB10, and SVOPL during Colorectal Cancer Tumorigenesis. International Journal of Genomics،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1165490

Modern Language Association (MLA)

Boot, Arnoud…[et al.]. Allelic Switching of DLX5, GRB10, and SVOPL during Colorectal Cancer Tumorigenesis. International Journal of Genomics No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1165490

American Medical Association (AMA)

Boot, Arnoud& Oosting, Jan& Doorn, Saskia& Ouahoud, Sarah& Ventayol Garcia, Marina& Ruano, Dina…[et al.]. Allelic Switching of DLX5, GRB10, and SVOPL during Colorectal Cancer Tumorigenesis. International Journal of Genomics. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1165490

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1165490