Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration Membrane
Joint Authors
Kamadjaja, David Buntoro
Rizqiawan, Andra
Harijadi, Achmad
Soesilawati, Pratiwi
Wahyuni, Eny
Maulidah, Nurul
Fauzi, Akhsanal
Rah Ayu, Fika
Simanjuntak, Roberto
Soesanto, R.
Asmara, Djodi
Agus, Peter
Pramono, Coen
Source
International Journal of Dentistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Bovine pericardium collagen membrane (BPCM) had been widely used in guided bone regeneration (GBR) whose manufacturing process usually required chemical cross-linking to prolong its biodegradation.
However, cross-linking of collagen fibrils was associated with poorer tissue integration and delayed vascular invasion.
Objective.
This study evaluated the potential of bovine cortical bone collagen membrane for GBR by evaluating its antigenicity potential, cytotoxicity, immune and tissue response, and biodegradation behaviors.
Material and Methods.
Antigenicity potential of demineralized freeze-dried bovine cortical bone membrane (DFDBCBM) was done with histology-based anticellularity evaluation, while cytotoxicity was analyzed using MTT Assay.
Evaluation of immune response, tissue response, and biodegradation was done by randomly implanting DFDBCBM and BPCM in rat’s subcutaneous dorsum.
Samples were collected at 2, 5, and 7 days and 7, 14, 21, and 28 days for biocompatibility and tissue response-biodegradation study, respectively.
Result.
DFDBCBM, histologically, showed no retained cells; however, it showed some level of in vitro cytotoxicity.
In vivo study exhibited increased immune response to DFDBCBM in early healing phase; however, normal tissue response and degradation rate were observed up to 4 weeks after DFDBCBM implantation.
Conclusion.
Demineralized freeze-dried bovine cortical bone membrane showed potential for clinical application; however, it needs to be optimized in its biocompatibility to fulfill all requirements for GBR membrane.
American Psychological Association (APA)
Kamadjaja, David Buntoro& Harijadi, Achmad& Soesilawati, Pratiwi& Wahyuni, Eny& Maulidah, Nurul& Fauzi, Akhsanal…[et al.]. 2017. Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration Membrane. International Journal of Dentistry،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1165638
Modern Language Association (MLA)
Kamadjaja, David Buntoro…[et al.]. Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration Membrane. International Journal of Dentistry No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1165638
American Medical Association (AMA)
Kamadjaja, David Buntoro& Harijadi, Achmad& Soesilawati, Pratiwi& Wahyuni, Eny& Maulidah, Nurul& Fauzi, Akhsanal…[et al.]. Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration Membrane. International Journal of Dentistry. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1165638
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1165638