Positive Selection of Squalene Synthase in Cucurbitaceae Plants
Joint Authors
Liu, Yong
Chen, Qicong
Ma, Chengtong
Qian, Jieying
Chao, Naixia
Wu, Yaosheng
Zhang, Yangmei
Luo, Yu
Cai, Danzhao
Source
International Journal of Genomics
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-09
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Triterpenoid saponins are secondary metabolites synthesized through isoprenoid pathways in plants.
Cucurbitaceae represent an important plant family in which many species contain cucurbitacins as secondary metabolites synthesized through isoprenoid and triterpenoid pathways.
Squalene synthase (SQS) is required for the biosynthesis of isoprenoids, but the forces driving the evolution of SQS remain undetermined.
In this study, 10 SQS cDNA sequences cloned from 10 species of Cucurbitaceae and 49 sequences of SQS downloaded from GenBank and UniProt databases were analyzed in a phylogenetic framework to identify the evolutionary forces for functional divergence.
Through phylogenetic construction and positive selection analysis, we found that SQS sequences are under positive selection.
The sites of positive selection map to functional and transmembrane domains.
180L, 189S, 194S, 196S, 265I, 289P, 389P, 390T, 407S, 408A, 410R, and 414N were identified as sites of positive selection that are important during terpenoid synthesis and map to transmembrane domains.
196S and 407S are phosphorylated and influence SQS catalysis and triterpenoid accumulation.
These results reveal that positive selection is an important evolutionary force for SQS in plants.
This provides new information into the molecular evolution of SQS within the Cucurbitaceae family.
American Psychological Association (APA)
Qian, Jieying& Liu, Yong& Ma, Chengtong& Chao, Naixia& Chen, Qicong& Zhang, Yangmei…[et al.]. 2019. Positive Selection of Squalene Synthase in Cucurbitaceae Plants. International Journal of Genomics،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1165667
Modern Language Association (MLA)
Qian, Jieying…[et al.]. Positive Selection of Squalene Synthase in Cucurbitaceae Plants. International Journal of Genomics No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1165667
American Medical Association (AMA)
Qian, Jieying& Liu, Yong& Ma, Chengtong& Chao, Naixia& Chen, Qicong& Zhang, Yangmei…[et al.]. Positive Selection of Squalene Synthase in Cucurbitaceae Plants. International Journal of Genomics. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1165667
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1165667