Transcriptome Analysis of Low-Temperature-Induced Breaking of Garlic Aerial Bulb Dormancy
Joint Authors
Dong, Yuhui
Guan, Mengjiao
Wang, Lixia
Yuan, Lei
Sun, Xiudong
Liu, Shiqi
Source
International Journal of Genomics
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-07
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
The long history of asexual reproduction of garlic using garlic cloves has resulted in virus accumulation and genetic depression.
Propagation of garlic seedlings by tissue culture can both eliminate viruses and improve breeding efficiency.
Aerial bulbs are the first-choice materials for breeding virus-free garlic seedlings under external conditions, but they show dormancy just like garlic bulbs.
However, low temperatures can quickly break dormancy.
In this research, we used a high-throughput sequencing method to sequence aerial bulbs during dormancy and after low-temperature-induced breaking of dormancy to screen out the key differentially expressed genes (DEGs) associated with low temperature and to provide a theoretical basis for exploring the molecular mechanism of low-temperature-induced breaking of aerial bulb dormancy.
The high-throughput transcriptome sequencing results showed that 6,675 DEGs were upregulated and 36,596 DEGs were downregulated in the aerial bulbs given low-temperature treatment.
Then, 19,507 DEGs were assigned KEGG annotations, among which most DEGs were annotated to the metabolism pathway (11,817 genes, accounting for 60.58%), followed by the genetic information processing pathway (4,521 genes, accounting for 23.18%).
The DEGs were mostly concentrated in pathways such as protein processing in the endoplasmic reticulum, plant-pathogen interaction, plant hormone signal transduction, and ribosome biogenesis in eukaryotes, with significant enrichment.
The key DEGs related to calcium signaling, hormonal signaling, and transcription factors were screened out, including CaM, CDPK, and CML in accessory pathways of calcium signaling; GA20ox, GAI1, and GA2ox in accessory pathways of hormonal signaling; and transcription factor genes such as MYB, AP2/ERF, bHLH, MADS, and bZIP.
qRT-PCR verification results were consistent with the sequencing results, indicating that the transcriptome sequencing data were accurate and reliable.
Our results provide a theoretical basis for breaking the dormancy of aerial bulbs with low-temperature treatment to produce virus-free seedlings and increase the output and quality of garlic.
American Psychological Association (APA)
Dong, Yuhui& Guan, Mengjiao& Wang, Lixia& Yuan, Lei& Sun, Xiudong& Liu, Shiqi. 2019. Transcriptome Analysis of Low-Temperature-Induced Breaking of Garlic Aerial Bulb Dormancy. International Journal of Genomics،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1165806
Modern Language Association (MLA)
Dong, Yuhui…[et al.]. Transcriptome Analysis of Low-Temperature-Induced Breaking of Garlic Aerial Bulb Dormancy. International Journal of Genomics No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1165806
American Medical Association (AMA)
Dong, Yuhui& Guan, Mengjiao& Wang, Lixia& Yuan, Lei& Sun, Xiudong& Liu, Shiqi. Transcriptome Analysis of Low-Temperature-Induced Breaking of Garlic Aerial Bulb Dormancy. International Journal of Genomics. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1165806
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1165806