DREB Genes from Common Bean (Phaseolus vulgaris L.) Show Broad to Specific Abiotic Stress Responses and Distinct Levels of Nucleotide Diversity
Joint Authors
Konzen, Enéas Ricardo
Recchia, Gustavo Henrique
Cassieri, Fernanda
Caldas, Danielle Gregorio Gomes
Berny Mier y Teran, Jorge C.
Gepts, Paul
Tsai, Siu Mui
Source
International Journal of Genomics
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-28, 28 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-02
Country of Publication
Egypt
No. of Pages
28
Main Subjects
Abstract EN
We analyzed the nucleotide variability and the expression profile of DREB genes from common bean, a crop of high economic and nutritional value throughout the world but constantly affected by abiotic stresses in cultivation areas.
As DREB genes have been constantly associated with abiotic stress tolerance, we systematically categorized 54 putative PvDREB genes distributed in the common bean genome.
It involved from AP2 domain location and amino acid conservation analysis (valine at the 14th position) to the identification of conserved motifs within peptide sequences representing six subgroups (A-1 to A-6) of PvDREB proteins.
Four genes (PvDREB1F, PvDREB2A, PvDREB5A, and PvDREB6B) were cloned and analyzed for their expression profiles under abiotic stresses and their nucleotide and amino acid diversity in genotypes of Andean and Mesoamerican origin, showing distinct patterns of expression and nucleotide variability.
PvDREB1F and PvDREB5A showed high relative inducibilities when genotypes of common bean were submitted to stresses by drought, salt, cold, and ABA.
PvDREB2A inducibility was predominantly localized to the stem under drought.
PvDREB6B was previously described as an A-2 (DREB2) gene, but a detailed phylogenetic analysis and its expression profile clearly indicated it belongs to group A-6.
PvDREB6B was found as a cold- and dehydration-responsive gene, mainly in leaves.
Interestingly, PvDREB6B also showed a high nucleotide and amino acid diversity within its coding region, in comparison to the others, implicating in several nonsynonymous amino acid substitutions between Andean and Mesoamerican genotypes.
The expression patterns and nucleotide diversity of each DREB found in this study revealed fundamental characteristics for further research aimed at understanding the molecular mechanisms associated with drought, salt, and cold tolerance in common bean, which could be performed based on association mapping and functional analyses.
American Psychological Association (APA)
Konzen, Enéas Ricardo& Recchia, Gustavo Henrique& Cassieri, Fernanda& Caldas, Danielle Gregorio Gomes& Berny Mier y Teran, Jorge C.& Gepts, Paul…[et al.]. 2019. DREB Genes from Common Bean (Phaseolus vulgaris L.) Show Broad to Specific Abiotic Stress Responses and Distinct Levels of Nucleotide Diversity. International Journal of Genomics،Vol. 2019, no. 2019, pp.1-28.
https://search.emarefa.net/detail/BIM-1165812
Modern Language Association (MLA)
Konzen, Enéas Ricardo…[et al.]. DREB Genes from Common Bean (Phaseolus vulgaris L.) Show Broad to Specific Abiotic Stress Responses and Distinct Levels of Nucleotide Diversity. International Journal of Genomics No. 2019 (2019), pp.1-28.
https://search.emarefa.net/detail/BIM-1165812
American Medical Association (AMA)
Konzen, Enéas Ricardo& Recchia, Gustavo Henrique& Cassieri, Fernanda& Caldas, Danielle Gregorio Gomes& Berny Mier y Teran, Jorge C.& Gepts, Paul…[et al.]. DREB Genes from Common Bean (Phaseolus vulgaris L.) Show Broad to Specific Abiotic Stress Responses and Distinct Levels of Nucleotide Diversity. International Journal of Genomics. 2019. Vol. 2019, no. 2019, pp.1-28.
https://search.emarefa.net/detail/BIM-1165812
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1165812