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Antimicrobial Activity of Lactic Acid Bacteria Starters against Acid Tolerant, Antibiotic Resistant, and Potentially Virulent E. coli Isolated from a Fermented Sorghum-Millet Beverage
Joint Authors
Byakika, Stellah
Mukisa, Ivan Muzira
Mugabi, Robert
Muyanja, Charles
Source
International Journal of Microbiology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Bacterial contamination of fermented foods is a serious global food safety challenge that requires effective control strategies.
This study characterized presumptive E.
coli isolated from Obushera, a traditional fermented cereal beverage from Uganda.
Thereafter, the antimicrobial effect of lactic acid bacteria (LAB) previously isolated from Obushera, against the E.
coli, was examined.
The presumptive E.
coli was incubated in brain heart infusion broth (pH = 3.6) at 25°C for 48 h.
The most acid-stable strains were clustered using (GTG)5 rep-PCR fingerprinting and identified using 16S rRNA sequencing.
E.
coli was screened for Shiga toxins (Stx 1 and Stx 2) and Intimin (eae) virulence genes as well as antibiotic resistance.
The spot-on-the-lawn method was used to evaluate antimicrobial activity.
Eighteen isolates were acid stable and are identified as E.
coli, Shigella, and Lysinibacillus.
The Stx 2 gene and antibiotic resistance were detected in some E.
coli isolates.
The LAB were antagonistic against the E.
coli.
Lactic acid bacteria from traditional fermented foods can be applied in food processing to inhibit pathogens.
Obushera lactic acid bacteria could be used to improve the safety of fermented foods.
American Psychological Association (APA)
Byakika, Stellah& Mukisa, Ivan Muzira& Mugabi, Robert& Muyanja, Charles. 2019. Antimicrobial Activity of Lactic Acid Bacteria Starters against Acid Tolerant, Antibiotic Resistant, and Potentially Virulent E. coli Isolated from a Fermented Sorghum-Millet Beverage. International Journal of Microbiology،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1166175
Modern Language Association (MLA)
Byakika, Stellah…[et al.]. Antimicrobial Activity of Lactic Acid Bacteria Starters against Acid Tolerant, Antibiotic Resistant, and Potentially Virulent E. coli Isolated from a Fermented Sorghum-Millet Beverage. International Journal of Microbiology No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1166175
American Medical Association (AMA)
Byakika, Stellah& Mukisa, Ivan Muzira& Mugabi, Robert& Muyanja, Charles. Antimicrobial Activity of Lactic Acid Bacteria Starters against Acid Tolerant, Antibiotic Resistant, and Potentially Virulent E. coli Isolated from a Fermented Sorghum-Millet Beverage. International Journal of Microbiology. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1166175
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166175