Androgen Receptor Localizes to Plasma Membrane by Binding to Caveolin-1 in Mouse Sertoli Cells
Joint Authors
Gui, Yaoting
Li, Minghua
Deng, Qiong
Wu, Yong
Zhang, Zeng
Wang, Yue
Liang, Hui
Source
International Journal of Endocrinology
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The nonclassical androgen signaling pathway translates signals into alterations in cellular function within minutes, and this action is proposed to be mediated by an androgen receptor (AR) localized to the plasma membrane.
This study was designed to determine the mechanism underlying the membrane association of androgen receptor in TM4 cells, a mouse Sertoli cell line.
Western blot analysis indicated testosterone-induced AR translocation to the cell membrane.
Data from coimmunoprecipitation indicated that AR is associated with caveolin-1, and testosterone enhanced this association.
Knockdown of caveolin-1 by shRNA decreased the amount of AR localized to membrane fraction and prevented AR membrane trafficking after being exposed to testosterone at physiological concentration.
The palmitoylation inhibitor 2-bromopalmitate decreased AR membrane localization in basal condition and completely blocked testosterone-induced AR translocation to membrane fraction.
These data suggested that AR localized to membrane fraction by binding with caveolin-1 through palmitoylation of the cysteine residue.
This study provided a new evidence for AR membrane localization and its application for clarifying the nonclassical signaling pathway of androgens.
American Psychological Association (APA)
Deng, Qiong& Wu, Yong& Zhang, Zeng& Wang, Yue& Li, Minghua& Liang, Hui…[et al.]. 2017. Androgen Receptor Localizes to Plasma Membrane by Binding to Caveolin-1 in Mouse Sertoli Cells. International Journal of Endocrinology،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1166187
Modern Language Association (MLA)
Deng, Qiong…[et al.]. Androgen Receptor Localizes to Plasma Membrane by Binding to Caveolin-1 in Mouse Sertoli Cells. International Journal of Endocrinology No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1166187
American Medical Association (AMA)
Deng, Qiong& Wu, Yong& Zhang, Zeng& Wang, Yue& Li, Minghua& Liang, Hui…[et al.]. Androgen Receptor Localizes to Plasma Membrane by Binding to Caveolin-1 in Mouse Sertoli Cells. International Journal of Endocrinology. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1166187
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166187