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Determination of In Vitro Antimicrobial Activity of Five Sri Lankan Medicinal Plants against Selected Human Pathogenic Bacteria
Joint Authors
Hewawasam, Ruwani P.
De Zoysa, Manikkuwadura Hasara Nethmini
Rathnayake, Hasanga
Wijayaratne, Weerasinghe Mudiyanselage Dilip Gaya Bandara
Source
International Journal of Microbiology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Introduction.
Antibiotic resistance is one of the greatest threats of the 21st century.
Scientists search for potential antimicrobial sources that can cope with antibiotic resistance.
Plants used in traditional medicine can be identified as potential candidates for the synthesis of novel drug compounds to act against antibiotic-resistant bacteria.
Objective.
To determine the potential antimicrobial effects of ethanol, aqueous, and hexane extracts of five Sri Lankan medicinal plants against four human pathogens.
Methods.
Asparagus falcatus (tubers), Asteracantha longifolia (whole plant), Vetiveria zizanioides (roots), Epaltes divaricata (whole plant), and Coriandrum sativum (seeds) were used in the study.
Plant extracts were screened against four clinically important Gram-positive and Gram-negative bacterial strains, Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), and Klebsiella pneumoniae (ATCC 700603).
Antibacterial activity of plant extracts were monitored using the agar disc diffusion method.
Eight concentrations of each positive plant extract were used to determine the minimum inhibitory concentration (MIC) by 5-fold dilution of plant extracts yielding a serial dilution of the original extract.
Results.
Ethanol, aqueous, and hexane extracts of E.
divaricata gave the maximum zones of inhibition of 16.3 mm, 7.4 mm, and 13.7 mm and MIC values of 0.48 mg/ml, 1.2 mg/ml, and 1.6 mg/ml, respectively, against S.
aureus.
Ethanol and hexane extracts of V.
zizanioides gave the maximum zones of inhibition of 12.1 mm and 11.4 mm and MIC values 2.4 mg/ml and 0.003 mg/ml, respectively, against S.
aureus.
None of the other plants were effective against any microorganism used for the study.
Conclusions.
It can be concluded that E.
divaricata and V.
zizanioides crude ethanol, aqueous, and hexane extracts exhibited significant in vitro antibacterial activity against S.
aureus, and the active compounds isolated from them can be potential sources for the synthesis of antibacterial drugs.
American Psychological Association (APA)
De Zoysa, Manikkuwadura Hasara Nethmini& Rathnayake, Hasanga& Hewawasam, Ruwani P.& Wijayaratne, Weerasinghe Mudiyanselage Dilip Gaya Bandara. 2019. Determination of In Vitro Antimicrobial Activity of Five Sri Lankan Medicinal Plants against Selected Human Pathogenic Bacteria. International Journal of Microbiology،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1166286
Modern Language Association (MLA)
De Zoysa, Manikkuwadura Hasara Nethmini…[et al.]. Determination of In Vitro Antimicrobial Activity of Five Sri Lankan Medicinal Plants against Selected Human Pathogenic Bacteria. International Journal of Microbiology No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1166286
American Medical Association (AMA)
De Zoysa, Manikkuwadura Hasara Nethmini& Rathnayake, Hasanga& Hewawasam, Ruwani P.& Wijayaratne, Weerasinghe Mudiyanselage Dilip Gaya Bandara. Determination of In Vitro Antimicrobial Activity of Five Sri Lankan Medicinal Plants against Selected Human Pathogenic Bacteria. International Journal of Microbiology. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1166286
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166286