Isolation of Polygalacturonase-Producing Bacterial Strain from Tomatoes (Lycopersicon esculentum Mill.)
Joint Authors
Obafemi, Yemisi Dorcas
Ajayi, Adesola Adetutu
Taiwo, Olugbenga Samson
Olorunsola, Shade John
Isibor, Patrick Omoregie
Source
International Journal of Microbiology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Polygalacturonase (EC 3.2.1.15) enzyme aids in microbial spoilage of fruits and vegetables.
It is very important to find economical ways to producing the enzyme so as to achieve maximum yield in industries due to its use at different areas of production process.
Methods.
Isolation of polygalacturonase-producing bacterial strain from tomatoes (Lycopersicon esculentum Mill.) was studied.
Polygalacturonase-producing bacterial strains were isolated and screened from tomatoes stored at normal laboratory temperature (25 ± 2°C).
They were identified based on their morphological, biochemical, and molecular characteristics.
The enzyme produced was partially purified by the ammonium sulphate precipitation method.
Molecular weights and optimum conditions for best enzyme activity were obtained by SDS PAGE technique.
Results.
Five bacterial isolates resulted after screening.
Bacterial strain code B5 showed highest polygalacturonase activity.
Optimum conditions for polygalacturonase PEC B5 were maintained at pH 4.5; temperature 35°C; substrate concentration 0.3 mg/ml, and best activity at less than 5 min of heating.
The enzyme PEC B5 was found to weigh 65 kDa and 50 kDa for crude and partially purified aliquots, respectively.
The result of 16S rRNA gene sequencing revealed bacterial strain code B5 as Enterobacter tabaci NR146667 having 79% similarity with the NCBI GenBank.
Conclusion.
Microorganisms should be developed for large-scale production of enzymes in developing countries.
American Psychological Association (APA)
Obafemi, Yemisi Dorcas& Ajayi, Adesola Adetutu& Taiwo, Olugbenga Samson& Olorunsola, Shade John& Isibor, Patrick Omoregie. 2019. Isolation of Polygalacturonase-Producing Bacterial Strain from Tomatoes (Lycopersicon esculentum Mill.). International Journal of Microbiology،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1166295
Modern Language Association (MLA)
Obafemi, Yemisi Dorcas…[et al.]. Isolation of Polygalacturonase-Producing Bacterial Strain from Tomatoes (Lycopersicon esculentum Mill.). International Journal of Microbiology No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1166295
American Medical Association (AMA)
Obafemi, Yemisi Dorcas& Ajayi, Adesola Adetutu& Taiwo, Olugbenga Samson& Olorunsola, Shade John& Isibor, Patrick Omoregie. Isolation of Polygalacturonase-Producing Bacterial Strain from Tomatoes (Lycopersicon esculentum Mill.). International Journal of Microbiology. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1166295
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166295