Gas Chromatography Coupled to High Resolution Time-of-Flight Mass Spectrometry as a High-Throughput Tool for Characterizing Geochemical Biomarkers in Sediments
Joint Authors
Bataglion, Giovana Anceski
Koolen, Hector Henrique Ferreira
Weber, Rolf Roland
Klitzke, Clécio Fernando
Binkley, Joe
Patrick, Jeffrey
de Albergaria-Barbosa, Ana Cecília Rizatti
Bícego, Márcia Caruso
Eberlin, Marcos Nogueira
Source
International Journal of Analytical Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The performance of gas chromatography coupled to high-resolution time-of-flight mass spectrometry (GC-HRTofMS) for characterizing geochemical biomarkers from sediment samples was evaluated.
Two approaches to obtain the geochemical biomarkers were tested: (1) extraction with organic solvent and subsequent derivatization and (2) in-situ derivatization thermal desorption.
Results demonstrated that both approaches can be conveniently applied for simultaneous characterization of many geochemical biomarkers (alkanes, alkanols, sterols, and fatty acids), avoiding conventional time-consuming purification procedures.
GC-HRTofMS reduces both sample preparation time and the number of chromatographic runs compared to traditional methodologies used in organic geochemistry.
Particularly, the approach based on in-situ derivatization thermal desorption represents a very simple method that can be performed in-line employing few milligrams of sediment, eliminating the need for any sample preparation and solvent use.
The high resolving power (m/Δm50% 25,000) and high mass accuracy (error ≤ 1 ppm) offered by the “zig-zag” time-of-flight analyzer were indispensable to resolve the complexity of the total ion chromatograms, representing a high-throughput tool.
Extracted ion chromatograms using exact m/z were useful to eliminate many isobaric interferences and to increase significantly the signal to noise ratio.
Characteristic fragment ions allowed the identification of homologous series, such as alkanes, alkanols, fatty acids, and sterols.
Polycyclic aromatic hydrocarbons were also identified in the samples by their molecular ions.
The characterization of geochemical biomarkers along a sedimentary core collected in the area of Valo Grande Channel (Cananéia-Iguape Estuarine-Lagunar System (São Paulo, Brazil)) provided evidences of environmental changes.
Sediments deposited before opening of channel showed dominance of biomarkers from mangrove vegetation, whereas sediments of the pos-opening period showed an increase of biomarkers from aquatic macrophyte (an invasive vegetation).
American Psychological Association (APA)
Koolen, Hector Henrique Ferreira& Klitzke, Clécio Fernando& Binkley, Joe& Patrick, Jeffrey& de Albergaria-Barbosa, Ana Cecília Rizatti& Weber, Rolf Roland…[et al.]. 2018. Gas Chromatography Coupled to High Resolution Time-of-Flight Mass Spectrometry as a High-Throughput Tool for Characterizing Geochemical Biomarkers in Sediments. International Journal of Analytical Chemistry،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1166317
Modern Language Association (MLA)
Koolen, Hector Henrique Ferreira…[et al.]. Gas Chromatography Coupled to High Resolution Time-of-Flight Mass Spectrometry as a High-Throughput Tool for Characterizing Geochemical Biomarkers in Sediments. International Journal of Analytical Chemistry No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1166317
American Medical Association (AMA)
Koolen, Hector Henrique Ferreira& Klitzke, Clécio Fernando& Binkley, Joe& Patrick, Jeffrey& de Albergaria-Barbosa, Ana Cecília Rizatti& Weber, Rolf Roland…[et al.]. Gas Chromatography Coupled to High Resolution Time-of-Flight Mass Spectrometry as a High-Throughput Tool for Characterizing Geochemical Biomarkers in Sediments. International Journal of Analytical Chemistry. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1166317
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166317