Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis
Joint Authors
González-Ortiz, Luis J.
Cid-Hernández, Margarita
López Dellamary-Toral, Fernando Antonio
Sánchez-Peña, María Judith
Pacheco Moisés, Fermín Paul
Source
International Journal of Analytical Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Spirulina platensis contains several biologically active compounds, some of them with antioxidant activity.
Nevertheless, not all of these compounds have been identified to date.
As a first step to achieving such identification, a methodology to perform two-dimensional thin layer chromatography bioautographies on silica gel thin layer chromatography plates was proposed.
Starting with a reference binary system, 5 other binary systems were tested, in which the relative polarity was systematically increased.
To further improve the separation behavior, a phase modifier (NH4OH) was used.
The best separation results were obtained with the isopropyl alcohol/ethyl acetate/NH4OH ternary system.
This experimental system allowed four well-resolved spots showing antioxidant activity as well as two additional areas with mixtures containing antioxidant compounds.
Although the proposed methodology was designed with a specific application, it would be predictable that its field of use could be considerably greater, making the convenient modifications on the solvent polarity and “masking level” produced by the ammonium derivatives.
American Psychological Association (APA)
Cid-Hernández, Margarita& López Dellamary-Toral, Fernando Antonio& González-Ortiz, Luis J.& Sánchez-Peña, María Judith& Pacheco Moisés, Fermín Paul. 2018. Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis. International Journal of Analytical Chemistry،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1166395
Modern Language Association (MLA)
Cid-Hernández, Margarita…[et al.]. Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis. International Journal of Analytical Chemistry No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1166395
American Medical Association (AMA)
Cid-Hernández, Margarita& López Dellamary-Toral, Fernando Antonio& González-Ortiz, Luis J.& Sánchez-Peña, María Judith& Pacheco Moisés, Fermín Paul. Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis. International Journal of Analytical Chemistry. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1166395
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166395