Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography
Joint Authors
Choochuay, Sasiprapa
Phakam, Jutamas
Jala, Prakorn
Maneeboon, Thanapoom
Tansakul, Natthasit
Source
International Journal of Analytical Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A reliable and rapid method has been developed for the determination of aflatoxin B1 (AFB1) in four kinds of feedstuffs comprising broken rice, peanuts, corn, and fishmeal.
A sample preparation was carried out based on the QuEChERS method with the exclusion of the clean-up step.
In this study, AFB1 was extracted using acetonitrile/methanol (40/60 v/v), followed by partitioning with sodium chloride and magnesium sulfate.
High-performance liquid chromatography with precolumn derivatization and fluorescence detection was performed.
The coefficients of determination were greater than 0.9800.
Throughout the developed method, the recovery of all feedstuffs achieved a range of 82.50-109.85% with relative standard deviation lower than 11% for all analytes at a concentration of 20-100 ng/g.
The limit of detection (LOD) ranged from 0.2 to 1.2 ng/g and limit of quantitation (LOQ) ranged from 0.3 to 1.5 ng/g.
The validated method was successfully applied to a total of 120 samples.
The occurrence of AFB1 contamination was found at the following concentrations: in broken rice (0.44-2.33ng/g), peanut (3.97-106.26ng/g), corn (0.88-50.29 ng/g), and fishmeal (1.06-10.35 ng/g).
These results indicate that the proposed method may be useful for regularly monitoring AFB1 contamination in feedstuffs.
American Psychological Association (APA)
Choochuay, Sasiprapa& Phakam, Jutamas& Jala, Prakorn& Maneeboon, Thanapoom& Tansakul, Natthasit. 2018. Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography. International Journal of Analytical Chemistry،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1166400
Modern Language Association (MLA)
Choochuay, Sasiprapa…[et al.]. Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography. International Journal of Analytical Chemistry No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1166400
American Medical Association (AMA)
Choochuay, Sasiprapa& Phakam, Jutamas& Jala, Prakorn& Maneeboon, Thanapoom& Tansakul, Natthasit. Determination of Aflatoxin B1 in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography. International Journal of Analytical Chemistry. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1166400
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166400