Preparation of Ampicillin Surface Molecularly Imprinted Polymers for Its Selective Recognition of Ampicillin in Eggs Samples
Joint Authors
Gong, Bolin
Tian, Yang
Wang, Yue
Wu, Shanshan
Sun, Zhian
Source
International Journal of Analytical Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-15
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Surface-imprinted polymers (MIPs) microspheres with the ability to specifically recognize water-soluble molecules were prepared using self-made monodisperse porous poly(chloromethylstyrene-co-divinylbenzene) beads as the solid-phase matrix and ampicillin (AMP) as the template molecule.
MIPs were synthesized using different template molecule: monomer: crosslinker ratios and the optimum preparation ratio were obtained by measuring adsorption.
The maximum equilibrium amount of adsorption by the MIPs reached 115.62 mg/g.
Scatchard analysis indicated that the MIPs contained two types of recognition sites: specific and nonspecific.
Based on the adsorption kinetics, adsorption equilibrium was reached after 30 minutes.
Penicillin G, amoxicillin, and sulbactam acid were used as competitive molecules to research the selective adsorption capacity of the MIPs.
The imprinted material was found to have good selectivity with selectivity coefficients for penicillin G, amoxicillin, and sulbactam acid of 5.74, 6.83, and 7.25, respectively.
The MIPs were used as solid-phase extraction filler, resulting in successful enrichment and separation of ampicillin residue from egg samples.
Standard addition recovery experiments revealed that recovery was good with recoveries from the spiked samples ranging from 91.5 to 94.9% and relative standard deviations from 3.6 to 4.2%.
The solid-phase extraction MIPs microcolumn was reused 10 times, where it maintained a recovery rate of over 80%.
This work presents a sensitive, fast, and convenient method for the determination of trace ampicillin in food samples.
American Psychological Association (APA)
Tian, Yang& Wang, Yue& Wu, Shanshan& Sun, Zhian& Gong, Bolin. 2018. Preparation of Ampicillin Surface Molecularly Imprinted Polymers for Its Selective Recognition of Ampicillin in Eggs Samples. International Journal of Analytical Chemistry،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1166444
Modern Language Association (MLA)
Tian, Yang…[et al.]. Preparation of Ampicillin Surface Molecularly Imprinted Polymers for Its Selective Recognition of Ampicillin in Eggs Samples. International Journal of Analytical Chemistry No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1166444
American Medical Association (AMA)
Tian, Yang& Wang, Yue& Wu, Shanshan& Sun, Zhian& Gong, Bolin. Preparation of Ampicillin Surface Molecularly Imprinted Polymers for Its Selective Recognition of Ampicillin in Eggs Samples. International Journal of Analytical Chemistry. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1166444
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166444