![](/images/graphics-bg.png)
Development and Validation of an HPLC-MSMS Method for Rapid Simultaneous Determination of Cefprozil Diastereomers in Human Plasma
Joint Authors
He, Guodong
Mai, Liping
Wang, Xipei
Source
International Journal of Analytical Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Both cis- and trans-cefprozil have antimicrobial activity, but their potencies are quite different.
It is therefore necessary to develop a sensitive method to simultaneously determine both isomers for pharmacokinetic and bioequivalence studies.
Methods.
An LC-MS/MS method, using stable isotope-labeled cefprozil as the internal standard, was developed and validated.
The analytes were extracted from plasma by protein precipitation and separated on a reverse-phase C18 column using a gradient program consisting of 0.5% formic acid and acetonitrile within 4 min.
The mass spectrometry acquisition was performed with multiple reaction monitoring in positive ion mode using the respective [M+H]+ ions, m/z 391.2→114.0 for cefprozil and 395.0→114.5 for cefprozil-D4.
Results.
The calibration curves were linear over the ranges of 0.025–15 μg/mL for cis-cefprozil and 0.014–1.67 μg/mL for trans-cefprozil.
The accuracies for the cis and trans isomers of cefprozil were 93.1% and 103.0%, respectively.
The intra- and interassay precisions for the QC samples of the isomers were < 14.3%.
The intra- and interassay precisions at the LLOQ were < 16.5%.
Conclusions.
The method was sensitive and reproducible and was applied in a pilot pharmacokinetic study of healthy volunteers.
American Psychological Association (APA)
He, Guodong& Mai, Liping& Wang, Xipei. 2018. Development and Validation of an HPLC-MSMS Method for Rapid Simultaneous Determination of Cefprozil Diastereomers in Human Plasma. International Journal of Analytical Chemistry،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1166479
Modern Language Association (MLA)
He, Guodong…[et al.]. Development and Validation of an HPLC-MSMS Method for Rapid Simultaneous Determination of Cefprozil Diastereomers in Human Plasma. International Journal of Analytical Chemistry No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1166479
American Medical Association (AMA)
He, Guodong& Mai, Liping& Wang, Xipei. Development and Validation of an HPLC-MSMS Method for Rapid Simultaneous Determination of Cefprozil Diastereomers in Human Plasma. International Journal of Analytical Chemistry. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1166479
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166479