Reference Gene and Protein Expression Levels in Two Different NAFLD Mouse Models
Joint Authors
Carvalho, Carla Roberta de Oliveira
Araujo, Layanne C. C.
Bordin, Silvana
Source
Gastroenterology Research and Practice
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The expression levels of some reference genes and proteins are used for data normalization and quantification.
However, these levels can change in response to experimental conditions or treatments.
Aim.
The aim of this work was to evaluate reference gene and protein expression in models of nonalcoholic fatty liver disease, using mice fed with a high-fat diet (HFD) and mice that are genetically obese (ob/ob).
Main Methods.
Histological staining techniques were used to verify the morphology and quantify the amount of lipid droplets present in the liver.
Real-time polymerase chain reaction and immunoblotting were employed for monitoring protein expression and gene expression levels, respectively.
Key Finding.
The results showed that there was a substantial increase in the amount of lipid droplets in the livers of HFD and ob/ob animals when compared to the standard diet (SD) group.
There was an observed reduction in the expression of β-actin (10%), α-tubulin (6%), GAPDH (19%), and RPL3 (15%) genes when comparing the ob/ob group to the HFD group.
Additionally, the ob/ob mice displayed GAPDH protein levels that were substantially, but not significantly, reduced when compared to SD.
Significance.
It was concluded that there are slight differences in the expression levels of reference genes and proteins in these two NAFLD animal models, and researchers should consider these alterations when working with these models.
American Psychological Association (APA)
Araujo, Layanne C. C.& Bordin, Silvana& Carvalho, Carla Roberta de Oliveira. 2020. Reference Gene and Protein Expression Levels in Two Different NAFLD Mouse Models. Gastroenterology Research and Practice،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1166628
Modern Language Association (MLA)
Araujo, Layanne C. C.…[et al.]. Reference Gene and Protein Expression Levels in Two Different NAFLD Mouse Models. Gastroenterology Research and Practice No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1166628
American Medical Association (AMA)
Araujo, Layanne C. C.& Bordin, Silvana& Carvalho, Carla Roberta de Oliveira. Reference Gene and Protein Expression Levels in Two Different NAFLD Mouse Models. Gastroenterology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1166628
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166628