Silencing of Long Noncoding RNA LINC00324 Interacts with MicroRNA-3200-5p to Attenuate the Tumorigenesis of Gastric Cancer via Regulating BCAT1
Joint Authors
Wang, Shuang
Cheng, Yuanyuan
Yang, Pingping
Qin, Guang
Source
Gastroenterology Research and Practice
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-13
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Purpose.
This study was aimed at exploring the effect of long noncoding RNA LINC00324 (LINC00324) on gastric cancer (GC) and the potential molecular mechanisms.
Methods.
The expression of LINC00324 and miR-3200-5p in GC tissues and cells was detected by qRT-PCR.
LINC00324 was silenced in GC cells by transfection of si-LINC00324.
Then, the proliferation, migration, and invasion of GC cells were analyzed by MTT, wound healing, and transwell assays, respectively.
The interactions between LINC00324 and miR-3200-5p and between miR-3200-5p and BCAT1 were determined by a dual-luciferase reporter and/or RNA pull-down assay.
Results.
The expression of LINC00324 was upregulated in GC cells and tissues, but miR-3200-5p was downregulated.
Silencing of LINC00324 inhibited the proliferation, migration, and invasion of GC cells.
LINC00324 directly targeted miR-3200-5p, and miR-3200-5p directly targeted BCAT1.
si-LINC00324 negatively regulated BCAT1 expression via binding to miR-3200-5p.
Furthermore, silencing of LINC00324 reversed the promoting effects of BCAT1 on the proliferation, migration, and invasion of GC cells.
Conclusion.
Silencing of LINC00324 inhibited the proliferation, migration, and invasion of GC cells through regulating the miR-3200-5p/BCAT1 axis.
American Psychological Association (APA)
Wang, Shuang& Cheng, Yuanyuan& Yang, Pingping& Qin, Guang. 2020. Silencing of Long Noncoding RNA LINC00324 Interacts with MicroRNA-3200-5p to Attenuate the Tumorigenesis of Gastric Cancer via Regulating BCAT1. Gastroenterology Research and Practice،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1166810
Modern Language Association (MLA)
Wang, Shuang…[et al.]. Silencing of Long Noncoding RNA LINC00324 Interacts with MicroRNA-3200-5p to Attenuate the Tumorigenesis of Gastric Cancer via Regulating BCAT1. Gastroenterology Research and Practice No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1166810
American Medical Association (AMA)
Wang, Shuang& Cheng, Yuanyuan& Yang, Pingping& Qin, Guang. Silencing of Long Noncoding RNA LINC00324 Interacts with MicroRNA-3200-5p to Attenuate the Tumorigenesis of Gastric Cancer via Regulating BCAT1. Gastroenterology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1166810
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166810