Diagnostic Value of Plasma miR-181b, miR-196a, and miR-210 Combination in Pancreatic Cancer

Abstract EN

Purpose.

This study was aimed at investigating the roles of plasma miR-181b, miR-196a, and miR-210 in the diagnosis of pancreatic cancer (PC).

Methods.

Plasma samples were isolated from 40 patients with PC and 40 healthy individuals, respectively.

The expression of miR-181b, miR-196a, and miR-210 was detected by qRT-PCR.

The level of carbohydrate antigen 199 (CA199) was measured by an electrochemiluminescence (ECL) assay.

The receiver operating characteristic (ROC) curve was used to analyze the diagnostic value of miR-181b, miR-196a, miR-210, CA199, and their combinations in PC.

Results.

The expression of plasma miR-181b, miR-196a, and miR-210 was significantly upregulated in PC patients.

The plasma level of CA199 was also significantly increased in PC patients.

The expression of miR-181b, miR-196a, and miR-210 was closely associated with lymph node metastasis, clinical stage, and vascular invasion but not correlated with age, gender, and tumor size.

miR-181b, miR-196a, and miR-210 have lower AUC than CA199 in the diagnosis of PC.

miR-181b+miR-210 and miR-196a+miR-210 also have lower AUC than CA199.

It is worth noting that miR-181b+miR-196a+miR-210 has a higher AUC than CA199 in the diagnosis of PC.

Conclusion.

The combination of plasma miR-181b, miR-196a, and miR-210 had a good diagnostic value for PC.