MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wntβ-Cateninsignaling Pathway
Joint Authors
Wu, Xiaohong
Zhang, Yong
Guo, Junping
Yan, Xun
Shen, Li
Zhou, Jianping
Zhao, Jun
Zhuang, Min
Cao, Zhihong
Source
Gastroenterology Research and Practice
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-21
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Gastric cancer is one of the most frequently diagnosed cancer and poses a serious threat to health system in the world.
Upregulation of meningioma-associated protein (MAC30) has been found in many solid tumors and can regulate the proliferation, differentiation, and apoptosis of different tumor cells.
Quantitative polymerase chain reaction (qPCR) was used to detect the expression of MAC30 in 68 patients with gastric cancer and their adjacent tissues.
Lentiviral vector pGCSIL-shMAC30-GFP of the RNA interference (RNAi) of the MAC30 gene was transfected into gastric cancer BGC-823 cell line and the expression of lentivirus label protein GFP was observed via fluorescence microscope, while cell proliferation and apoptosis were determined with flow cytometry and MTT assay, respectively.
Also, related protein expressions on Wnt/β-catenin signaling pathway were analyzed by Western blot method.
The expression of MAC30 was abnormally elevated in gastric cancer tissues, while interfering of its expression could significantly inhibit the proliferation of gastric cancer BGC-823 cell line.
However, the promotion of apoptosis by mitochondrial pathway was mediated by Bax/Bcl-2 upregulation.
Present work showed the effect of downregulated MAC30 expression on proliferation and apoptosis of gastric cancer cell through Wnt/β-catenin signaling pathway.
Thus, this investigation provides an experimental basis for future development of chemotherapeutic agent on gastric cancer.
American Psychological Association (APA)
Wu, Xiaohong& Zhang, Yong& Guo, Junping& Yan, Xun& Shen, Li& Zhou, Jianping…[et al.]. 2020. MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wntβ-Cateninsignaling Pathway. Gastroenterology Research and Practice،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1166934
Modern Language Association (MLA)
Wu, Xiaohong…[et al.]. MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wntβ-Cateninsignaling Pathway. Gastroenterology Research and Practice No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1166934
American Medical Association (AMA)
Wu, Xiaohong& Zhang, Yong& Guo, Junping& Yan, Xun& Shen, Li& Zhou, Jianping…[et al.]. MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wntβ-Cateninsignaling Pathway. Gastroenterology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1166934
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166934