Jiaweishaoyao Decoction Alleviates DSS-Induced Ulcerative Colitis via Inhibiting Inflammation
Joint Authors
Qiao, Huixia
Huang, Yahui
Chen, Xiaoyan
Yang, Long
Wang, Yue
Xu, Rongrong
Source
Gastroenterology Research and Practice
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Purpose.
Jiaweishaoyao decoction (JWSYD) is a traditional prescription of Chinese medicine that is initially used for the treatment of diarrhea.
This study is aimed at investigating the effects of JWSYD on DSS-induced ulcerative colitis (UC).
Methods.
DSS-induced UC mice and LPS-induced RAW264.7 cells were used as the UC model in vivo and in vitro.
UC was assessed by body weight, disease activity index (DAI), colon length, spleen weight, and histopathological score (HE staining).
The levels of TNF-α, IL-1β, and IL-6 were analyzed by ELISA and qRT-PCR.
The levels of NLRP3 inflammasome- and NF-κB pathway-associated proteins were measured by western blot.
Results.
JWSYD alleviated DSS-induced UC in respect to body weight, DAI, colon length, spleen weight, and histopathological score.
JWSYD reduced the levels of TNF-α, IL-1β, and IL-6 in DSS-induced UC mice and the supernatants of LPS-induced RAW264.7 cells.
JWSYD suppressed the protein levels of inflammasome-associated proteins, including NLRP3, ASC1, Procaspase-1, Cleaved caspase-1, and Cleaved IL-1β in DSS-induced UC mice and LPS-induced RAW264.7 cells.
In addition, JWSYD suppressed the NF-κB pathway in vitro and in vivo.
Conclusion.
JWSYD alleviated DSS-induced UC via inhibiting the NLRP3 inflammasome and NF-κB pathway.
American Psychological Association (APA)
Qiao, Huixia& Huang, Yahui& Chen, Xiaoyan& Yang, Long& Wang, Yue& Xu, Rongrong. 2020. Jiaweishaoyao Decoction Alleviates DSS-Induced Ulcerative Colitis via Inhibiting Inflammation. Gastroenterology Research and Practice،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1166982
Modern Language Association (MLA)
Qiao, Huixia…[et al.]. Jiaweishaoyao Decoction Alleviates DSS-Induced Ulcerative Colitis via Inhibiting Inflammation. Gastroenterology Research and Practice No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1166982
American Medical Association (AMA)
Qiao, Huixia& Huang, Yahui& Chen, Xiaoyan& Yang, Long& Wang, Yue& Xu, Rongrong. Jiaweishaoyao Decoction Alleviates DSS-Induced Ulcerative Colitis via Inhibiting Inflammation. Gastroenterology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1166982
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166982