miRNA Expression Profile and Effect of Wenxin Granule in Rats with Ligation-Induced Myocardial Infarction
Joint Authors
Zhai, Jianying
Ai-Ming, Wu
Gao, Yonghong
Nie, Bo
Shang, Hongcai
Hai-Yan, Zhu
Zhao, Mingjing
Chai, Limin
Li-Xia, Lou
Zhang, Dong-mei
Source
International Journal of Genomics
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Wenxin Granule (WXKL) is a traditional Chinese medicine used for treatment of myocardial infarction (MI) and arrhythmias.
However, the genomic pathological mechanisms of MI and mechanisms of WXKL are largely unknown.
This study aims to investigate a comprehensive miRNA expression profile, and the predicted correlation pathways to be targeted by differentially expressed miRNAs in MI, and mechanisms of WXKL from a gene level.
MI rat model was established by a coronary artery ligation surgery.
miRNA expression microarrays were performed and the data were deposited in Gene Expression Omnibus (GEO number GSE95855).
And, pathway analysis was performed by using the DIANA-miRPath v3.0 online tool.
The expressions of miR-1, miR-133, Cx43, and Cx45 were detected by quantitative real-time PCR.
It was found that 35 differentially expressed miRNAs and 23 predicted pathways, including miR-1, miR-133, and gap junction pathway, are involved in the pathogenesis of MI.
And, WXKL increased the expressions of miR-1 and miR-133, while also increased the mRNA levels of Cx43 and Cx45, and, especially, recovered the Cx43/Cx45 ratio near to normal level.
The results suggest that regulatory effects on miR-1, miR-133, Cx43, and Cx45 might be a possible mechanism of WXKL in the treatment of MI at the gene level.
American Psychological Association (APA)
Ai-Ming, Wu& Li-Xia, Lou& Zhai, Jianying& Zhang, Dong-mei& Chai, Limin& Nie, Bo…[et al.]. 2017. miRNA Expression Profile and Effect of Wenxin Granule in Rats with Ligation-Induced Myocardial Infarction. International Journal of Genomics،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1166997
Modern Language Association (MLA)
Ai-Ming, Wu…[et al.]. miRNA Expression Profile and Effect of Wenxin Granule in Rats with Ligation-Induced Myocardial Infarction. International Journal of Genomics No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1166997
American Medical Association (AMA)
Ai-Ming, Wu& Li-Xia, Lou& Zhai, Jianying& Zhang, Dong-mei& Chai, Limin& Nie, Bo…[et al.]. miRNA Expression Profile and Effect of Wenxin Granule in Rats with Ligation-Induced Myocardial Infarction. International Journal of Genomics. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1166997
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166997