Comparison of Platelet-Rich Plasma-Impregnated Suture Material with Low and High Platelet Concentration to Improve Colonic Anastomotic Wound Healing in Rats
Joint Authors
Senem, Demiroz Ahu
Aydin, Mehmet Akif
Guler, Eray Metin
Aydin, Muhammet Fatih
Saglam, Gulcan
Source
Gastroenterology Research and Practice
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
This study was designed to investigate the impact of using suture material impregnated with platelet-rich plasma (PRP) in different platelet concentrations on colonic anastomotic wound healing in rats.
Methods.
A total of 24 Sprague Dawley female rats were separated into 3 groups (n=8 for each) including the control group (CON; standard vicryl suture repair), the low platelet concentrate PRP group (L-PRP; suture material impregnated with PRP containing average 2.7-fold (range, 2.0 to 3.1) higher amount of platelets vs.
control), and the high platelet concentrate PRP group (H-PRP; suture material impregnated with PRP containing average 5.1-fold (range, 4.8 to 5.4) higher amount of platelets vs.
control).
Rats were sacrificed on the postoperative 7th day for analysis of colonic anastomosis region including macroscopic observation, measurement of anastomotic bursting pressure (ABP), and the hydroxyproline levels and histopathological findings in colon tissue samples.
Results.
Total injury scores were significantly lower in the L-PRP and H-PRP groups than those in the control group (median (range) 13.00 (7.00) and 11.50 (6.00) vs.
15.50 (4.00), p<0.05 and p<0.01, respectively).
ABP values (180.00 (49.00) vs.
124.00 (62.00) and 121.00 (57.00) mmHg, p<0.001 for each) and tissue hydroxyproline levels (0.56 (0.37) vs.
0.25 (0.17) and 0.39 (0.10) μg/mg tissue, p<0.001 and p<0.05, respectively) were significantly higher in the L-PRP group as compared with those in the control and H-PRP groups.
Conclusion.
In conclusion, our findings revealed PRP application to colonic anastomosis sutures to promote the anastomotic healing process.
The platelet concentration of PRP seems to have a significant impact on the outcome with superior efficacy of L-PRP over H-PRP in terms of bursting pressures and collagen concentration at the anastomotic site.
American Psychological Association (APA)
Aydin, Mehmet Akif& Guler, Eray Metin& Senem, Demiroz Ahu & Aydin, Muhammet Fatih& Saglam, Gulcan. 2020. Comparison of Platelet-Rich Plasma-Impregnated Suture Material with Low and High Platelet Concentration to Improve Colonic Anastomotic Wound Healing in Rats. Gastroenterology Research and Practice،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1167004
Modern Language Association (MLA)
Aydin, Mehmet Akif…[et al.]. Comparison of Platelet-Rich Plasma-Impregnated Suture Material with Low and High Platelet Concentration to Improve Colonic Anastomotic Wound Healing in Rats. Gastroenterology Research and Practice No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1167004
American Medical Association (AMA)
Aydin, Mehmet Akif& Guler, Eray Metin& Senem, Demiroz Ahu & Aydin, Muhammet Fatih& Saglam, Gulcan. Comparison of Platelet-Rich Plasma-Impregnated Suture Material with Low and High Platelet Concentration to Improve Colonic Anastomotic Wound Healing in Rats. Gastroenterology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1167004
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167004