Methanolic Extract of Distemonanthus benthamianus (Caesalpiniaceae) Stem Bark Suppresses EthanolIndomethacin-Induced Chronic Gastric Injury in Rats
Joint Authors
Mbiantcha, Marius
Ateufack, Gilbert
Tsafack, Eric Gonzal
Atsamo, Albert Donatien
Yousseu Nana, William
Adjouzem, Carine Flore
Matah Marte, Vanessa Mba
Djuichou Nguemnang, Stéphanie Flore
Madjo Kouam, Yacine Karelle
Ngoufack Azanze, Elvira
Source
Gastroenterology Research and Practice
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-28
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Distemonanthus benthamianus (Caesalpiniaceae) is a plant from the Cameroon pharmacopoeia very widely used in the treatment of many pathologies among which are gastrointestinal disorders.
The main purpose of this study was to assess the healing properties of gastric ulcer from the methanolic extract of Distemonanthus benthamianus and its mechanisms of action.
The healing properties of gastric ulcers (chronic ulcer model induced by ethanol and indomethacin) were evaluated in vivo in adult male rats, while the mechanisms of action were evaluated in vitro by anti-inflammatory assay (protein denaturation, cyclooxygenase, and lipoxygenase assays) and immunomodulatory assay (ROS production (using technical chemiluminescence), cytokine (TNF-α, IL-1β, IL-6) production (using ELISA), proliferation of T cells (using liquid scintillation counter), and cytotoxicity (using MTT assay)).
The methanolic extract of Distemonanthus benthamianus inhibited protein denaturation (75.63%) and the activities of cyclooxygenase (78.92%) and 5-lipoxygenase (81.54%).
The extract also significantly (p<0.001) inhibited intracellular and extracellular ROS production and T cell proliferation and reduced significantly (p<0.01, p<0.001) TNF-α, IL-1β, IL-6, and PGE2 production.
At all doses (125, 250, and 500 mg/kg), the extract significantly reduces the ulceration index and the area of ulceration and significantly increases the mass of gastric mucus.
In addition, the extract significantly decreases the level of MDA, significantly increases the activities of catalase and glutathione, and then improves the hematological parameters in sick animals.
Histological micrographs show that in the presence of the extract, there is advanced reepithelialization with recovery of the ulcerated epithelium.
Thus, the extract of Distemonanthus benthamianus has healing properties against gastric ulcers which are associated with its anti-inflammatory, immunomodulatory, and antioxidant effects.
American Psychological Association (APA)
Matah Marte, Vanessa Mba& Ateufack, Gilbert& Mbiantcha, Marius& Atsamo, Albert Donatien& Adjouzem, Carine Flore& Djuichou Nguemnang, Stéphanie Flore…[et al.]. 2020. Methanolic Extract of Distemonanthus benthamianus (Caesalpiniaceae) Stem Bark Suppresses EthanolIndomethacin-Induced Chronic Gastric Injury in Rats. Gastroenterology Research and Practice،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1167043
Modern Language Association (MLA)
Matah Marte, Vanessa Mba…[et al.]. Methanolic Extract of Distemonanthus benthamianus (Caesalpiniaceae) Stem Bark Suppresses EthanolIndomethacin-Induced Chronic Gastric Injury in Rats. Gastroenterology Research and Practice No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1167043
American Medical Association (AMA)
Matah Marte, Vanessa Mba& Ateufack, Gilbert& Mbiantcha, Marius& Atsamo, Albert Donatien& Adjouzem, Carine Flore& Djuichou Nguemnang, Stéphanie Flore…[et al.]. Methanolic Extract of Distemonanthus benthamianus (Caesalpiniaceae) Stem Bark Suppresses EthanolIndomethacin-Induced Chronic Gastric Injury in Rats. Gastroenterology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1167043
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167043