Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata
Joint Authors
Feng, Bo
Fang, Yang
Xu, Zhibin
Xiang, Chao
Zhou, Chunhong
Jiang, Fei
Wang, Tao
Zhao, Hai
Source
International Journal of Genomics
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Lemnaceae (commonly called duckweed) is an aquatic plant ideal for quantitative analysis in plant sciences.
Several species of this family represent the smallest and fastest growing flowering plants.
Different ecotypes of the same species vary in their biochemical and physiological properties.
Thus, selecting of desirable ecotypes of a species is very important.
Here, we developed a simple and rapid molecular identification system for Spirodela polyrhiza and Landoltia punctata based on the sequence polymorphism.
First, several pairs of primers were designed and three markers were selected as good for identification.
After PCR amplification, DNA fragments (the combination of three PCR products) in different duckweeds were detected using capillary electrophoresis.
The high-resolution capillary electrophoresis displayed high identity to the sequencing results.
The combination of the PCR products containing several DNA fragments highly improved the identification frequency.
These results indicate that this method is not only good for interspecies identification but also ideal for intraspecies distinguishing.
Meanwhile, 11 haplotypes were found in both the S.
polyrhiza and L.
punctata ecotypes.
The results suggest that this marker system is useful for large-scale identification of duckweed and for the screening of desirable ecotypes to improve the diverse usage in duckweed utilization.
American Psychological Association (APA)
Feng, Bo& Fang, Yang& Xu, Zhibin& Xiang, Chao& Zhou, Chunhong& Jiang, Fei…[et al.]. 2017. Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata. International Journal of Genomics،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1167119
Modern Language Association (MLA)
Feng, Bo…[et al.]. Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata. International Journal of Genomics No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1167119
American Medical Association (AMA)
Feng, Bo& Fang, Yang& Xu, Zhibin& Xiang, Chao& Zhou, Chunhong& Jiang, Fei…[et al.]. Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata. International Journal of Genomics. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1167119
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167119