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The miRNA Pull Out Assay as a Method to Validate the miR-28-5p Targets Identified in Other Tumor Contexts in Prostate Cancer
Joint Authors
Rizzo, Milena
Berti, Gabriele
Russo, Francesco
Evangelista, Monica
Pellegrini, Marco
Rainaldi, Giuseppe
Source
International Journal of Genomics
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-20
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
miR-28-5p is an intragenic miRNA which is underexpressed in several tumor types showing a tumor suppressor (TS) activity.
Routinely, the known miR-28-5p targets are validated in specific tumor contexts but it is unclear whether these targets are also being regulated in other tumor types.
To this end, we adopted the miRNA pull out assay to capture the miR-28-5p targets in DU-145 prostate cancer (PCa) cells.
Firstly, we demonstrated that miR-28-5p acts as a TS-miRNA in PCa, affecting cell proliferation, survival, and apoptosis.
Secondly, we evaluated the enrichment of the 10 validated miR-28-5p targets in the pull out sample.
We showed that E2F6, TEX-261, MAPK1, MPL, N4BP1, and RAP1B but not BAG1, OTUB1, MAD2L1, and p21 were significantly enriched, suggesting that not all the miR-28-5p targets are regulated by this miRNA in PCa.
We then verified whether the miR-28-5p-interacting targets were regulated by this miRNA.
We selected E2F6, the most enriched target in the pull out sample, and demonstrated that miR-28-5p downregulated E2F6 at the protein level suggesting that our approach was effective.
In general terms, these findings support the miRNA pull out assay as a useful method to identify context-specific miRNA targets.
American Psychological Association (APA)
Rizzo, Milena& Berti, Gabriele& Russo, Francesco& Evangelista, Monica& Pellegrini, Marco& Rainaldi, Giuseppe. 2017. The miRNA Pull Out Assay as a Method to Validate the miR-28-5p Targets Identified in Other Tumor Contexts in Prostate Cancer. International Journal of Genomics،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1167129
Modern Language Association (MLA)
Rizzo, Milena…[et al.]. The miRNA Pull Out Assay as a Method to Validate the miR-28-5p Targets Identified in Other Tumor Contexts in Prostate Cancer. International Journal of Genomics No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1167129
American Medical Association (AMA)
Rizzo, Milena& Berti, Gabriele& Russo, Francesco& Evangelista, Monica& Pellegrini, Marco& Rainaldi, Giuseppe. The miRNA Pull Out Assay as a Method to Validate the miR-28-5p Targets Identified in Other Tumor Contexts in Prostate Cancer. International Journal of Genomics. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1167129
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167129