Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini Species
Joint Authors
Araújo, Naiara Pereira
Kuhn, Gustavo Campos Silva
Vieira, Flávia Nunes
Morcatty, Thaís Queiroz
Paglia, Adriano Pereira
Svartman, Marta
Source
International Journal of Genomics
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
South American Akodontini rodents are characterized by a large number of chromosome rearrangements.
Among them, the genus Akodon has been extensively analyzed with classical and molecular cytogenetics, which allowed the identification of a large number of intra- and interspecific chromosomal variation due to Robertsonian rearrangements, pericentric inversions, and heterochromatin additions/deletions.
In order to shed some light on the cause of these rearrangements, we comparatively analyzed the karyotypes of three Akodontini species, Akodon cursor (2n = 14, FN = 19), A.
montensis (2n = 24, FN = 42), and Necromys lasiurus (2n = 34, FN = 34), after GTG- and CBG-banding.
The karyotypes differed by Robertsonian rearrangements, pericentric inversions, centromere repositioning, and heterochromatin variation.
Genome comparisons were performed through interspecific fluorescent in situ hybridization (FISH) with total genomic DNAs of each species as probes (GISH).
Our results revealed considerable conservation of the euchromatic portions among the three karyotypes suggesting that they mostly differ in their heterochromatic regions.
FISH was also performed to assess the distribution of telomeric sequences, long and short interspersed repetitive elements (LINE-1 and B1 SINE) and of the endogenous retrovirus mysTR in the genomes of the three species.
The results led us to infer that transposable elements have played an important role in the enormous chromosome variation found in Akodontini.
American Psychological Association (APA)
Araújo, Naiara Pereira& Kuhn, Gustavo Campos Silva& Vieira, Flávia Nunes& Morcatty, Thaís Queiroz& Paglia, Adriano Pereira& Svartman, Marta. 2017. Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini Species. International Journal of Genomics،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1167154
Modern Language Association (MLA)
Araújo, Naiara Pereira…[et al.]. Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini Species. International Journal of Genomics No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1167154
American Medical Association (AMA)
Araújo, Naiara Pereira& Kuhn, Gustavo Campos Silva& Vieira, Flávia Nunes& Morcatty, Thaís Queiroz& Paglia, Adriano Pereira& Svartman, Marta. Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini Species. International Journal of Genomics. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1167154
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167154