Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers
Joint Authors
Cer, Regina Z.
Herrera-Galeano, J. Enrique
Frey, Kenneth G.
Schully, Kevin L.
Luu, Truong V.
Pesce, John
Mokashi, Vishwesh P.
Keane-Myers, Andrea M.
Bishop-Lilly, Kimberly A.
Source
International Journal of Genomics
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Increasing evidence that microRNAs (miRNAs) play important roles in the immune response against infectious agents suggests that miRNA might be exploitable as signatures of exposure to specific infectious agents.
In order to identify potential early miRNA biomarkers of bacterial infections, human peripheral blood mononuclear cells (hPBMCs) were exposed to two select agents, Burkholderia pseudomallei K96243 and Francisella tularensis SHU S4, as well as to the nonpathogenic control Escherichia coli DH5α.
RNA samples were harvested at three early time points, 30, 60, and 120 minutes postexposure, then sequenced.
RNAseq analyses identified 87 miRNAs to be differentially expressed (DE) in a linear fashion.
Of these, 31 miRNAs were tested using the miScript miRNA qPCR assay.
Through RNAseq identification and qPCR validation, we identified differentially expressed miRNA species that may be involved in the early response to bacterial infections.
Based upon its upregulation at early time points postexposure in two different individuals, hsa-mir-30c-5p is a miRNA species that could be studied further as a potential biomarker for exposure to these gram-negative intracellular pathogens.
Gene ontology functional analyses demonstrated that programmed cell death is the first ranking biological process associated with miRNAs that are upregulated in F.
tularensis-exposed hPBMCs.
American Psychological Association (APA)
Cer, Regina Z.& Herrera-Galeano, J. Enrique& Frey, Kenneth G.& Schully, Kevin L.& Luu, Truong V.& Pesce, John…[et al.]. 2017. Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers. International Journal of Genomics،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1167176
Modern Language Association (MLA)
Cer, Regina Z.…[et al.]. Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers. International Journal of Genomics No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1167176
American Medical Association (AMA)
Cer, Regina Z.& Herrera-Galeano, J. Enrique& Frey, Kenneth G.& Schully, Kevin L.& Luu, Truong V.& Pesce, John…[et al.]. Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers. International Journal of Genomics. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1167176
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167176