Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) and Molecular Marker Development
Joint Authors
Ding, Qian
Li, Jingjuan
Wang, Fengde
Zhang, Yihui
Li, Huayin
Gao, Jianwei
Liu, Lifeng
Source
International Journal of Genomics
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Due to the visual appearance and high carotenoid content, orange inner leaves are a desirable trait for the Chinese cabbage.
To understand the molecular mechanism underlying the formation of orange inner leaves, the BrCRTISO (Bra031539) gene, as the Br-or candidate gene, was analyzed among the white and orange varieties, and 7 single nucleotide polymorphisms (SNPs) were identified.
However, only one SNP (C952 to T952) altered the amino acid sequence, resulting in a mutation from Leu318 to Phe318 in the orange varieties.
Additionally, we analyzed differentially expressed genes (DEGs) between the orange and white F2 individuals (14-401 × 14-490) and found four downregulated genes were involved in the carotenoid biosynthesis pathway, which may lead to the accumulation of prolycopene and other carotenoid pigments in the orange inner leaves.
In addition, we developed a novel InDel marker in the first intron, which cosegregates with the phenotypes of orange color inner leaves.
In conclusion, these findings enhance our understanding of the underlying mechanism of pigment accumulation in the inner leaves of the Chinese cabbage.
Additionally, the SNP (C952 to T952) and the InDel marker will facilitate the marker-assisted selection during Chinese cabbage breeding.
American Psychological Association (APA)
Li, Jingjuan& Zhang, Yihui& Ding, Qian& Li, Huayin& Liu, Lifeng& Wang, Fengde…[et al.]. 2017. Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) and Molecular Marker Development. International Journal of Genomics،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1167206
Modern Language Association (MLA)
Li, Jingjuan…[et al.]. Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) and Molecular Marker Development. International Journal of Genomics No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1167206
American Medical Association (AMA)
Li, Jingjuan& Zhang, Yihui& Ding, Qian& Li, Huayin& Liu, Lifeng& Wang, Fengde…[et al.]. Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) and Molecular Marker Development. International Journal of Genomics. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1167206
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167206