The Draft Genome and Transcriptome of the Atlantic Horseshoe Crab, Limulus polyphemus
Joint Authors
Simpson, Stephen D.
Ramsdell, Jordan S.
Watson III, Winsor H.
Chabot, Christopher C.
Source
International Journal of Genomics
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-06
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The horseshoe crab, Limulus polyphemus, exhibits robust circadian and circatidal rhythms, but little is known about the molecular mechanisms underlying those rhythms.
In this study, horseshoe crabs were collected during the day and night as well as high and low tides, and their muscle and central nervous system tissues were processed for genome and transcriptome sequencing, respectively.
The genome assembly resulted in 7.4 × 10 5 contigs with N50 of 4,736, while the transcriptome assembly resulted in 9.3 × 10 4 contigs and N50 of 3,497.
Analysis of functional completeness by the identification of putative universal orthologs suggests that the transcriptome has three times more total expected orthologs than the genome.
Interestingly, RNA-Seq analysis indicated no statistically significant changes in expression level for any circadian core or accessory gene, but there was significant cycling of several noncircadian transcripts.
Overall, these assemblies provide a resource to investigate the Limulus clock systems and provide a large dataset for further exploration into the taxonomy and biology of the Atlantic horseshoe crab.
American Psychological Association (APA)
Simpson, Stephen D.& Ramsdell, Jordan S.& Watson III, Winsor H.& Chabot, Christopher C.. 2017. The Draft Genome and Transcriptome of the Atlantic Horseshoe Crab, Limulus polyphemus. International Journal of Genomics،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1167232
Modern Language Association (MLA)
Simpson, Stephen D.…[et al.]. The Draft Genome and Transcriptome of the Atlantic Horseshoe Crab, Limulus polyphemus. International Journal of Genomics No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1167232
American Medical Association (AMA)
Simpson, Stephen D.& Ramsdell, Jordan S.& Watson III, Winsor H.& Chabot, Christopher C.. The Draft Genome and Transcriptome of the Atlantic Horseshoe Crab, Limulus polyphemus. International Journal of Genomics. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1167232
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167232