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Preparing and Analyzing Expressed Sequence Tags (ESTs) Library for the Mammary Tissue of Local Turkish Kivircik Sheep
Joint Authors
Ozdemir Ozgenturk, Nehir
Omeroglu Ulu, Zehra
Ulu, Salih
Un, Cemal
Ozdem Oztabak, Kemal
Altunatmaz, Kemal
Source
International Journal of Genomics
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-23
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Kivircik sheep is an important local Turkish sheep according to its meat quality and milk productivity.
The aim of this study was to analyze gene expression profiles of both prenatal and postnatal stages for the Kivircik sheep.
Therefore, two different cDNA libraries, which were taken from the same Kivircik sheep mammary gland tissue at prenatal and postnatal stages, were constructed.
Total 3072 colonies which were randomly selected from the two libraries were sequenced for developing a sheep ESTs collection.
We used Phred/Phrap computer programs for analysis of the raw EST and readable EST sequences were assembled with the CAP3 software.
Putative functions of all unique sequences and statistical analysis were determined by Geneious software.
Total 422 ESTs have over 80% similarity to known sequences of other organisms in NCBI classified by Panther database for the Gene Ontology (GO) category.
By comparing gene expression profiles, we observed some putative genes that may be relative to reproductive performance or play important roles in milk synthesis and secretion.
A total of 2414 ESTs have been deposited to the NCBI GenBank database (GW996847–GW999260).
EST data in this study have provided a new source of information to functional genome studies of sheep.
American Psychological Association (APA)
Ozdemir Ozgenturk, Nehir& Omeroglu Ulu, Zehra& Ulu, Salih& Un, Cemal& Ozdem Oztabak, Kemal& Altunatmaz, Kemal. 2017. Preparing and Analyzing Expressed Sequence Tags (ESTs) Library for the Mammary Tissue of Local Turkish Kivircik Sheep. International Journal of Genomics،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1167286
Modern Language Association (MLA)
Ozdemir Ozgenturk, Nehir…[et al.]. Preparing and Analyzing Expressed Sequence Tags (ESTs) Library for the Mammary Tissue of Local Turkish Kivircik Sheep. International Journal of Genomics No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1167286
American Medical Association (AMA)
Ozdemir Ozgenturk, Nehir& Omeroglu Ulu, Zehra& Ulu, Salih& Un, Cemal& Ozdem Oztabak, Kemal& Altunatmaz, Kemal. Preparing and Analyzing Expressed Sequence Tags (ESTs) Library for the Mammary Tissue of Local Turkish Kivircik Sheep. International Journal of Genomics. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1167286
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167286