In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in Ethiopia
Joint Authors
Sbhatu, Desta Berhe
Weldemichael, Micheale Yifter
Gebru, Haftay Abadi
Bayratsion, Yemane Tsehaye
Abraha, Girmay Gebresamuel
Juhar, Hagos Mohammedseid
Birhan Kassa, Abraha
Sibhatu, Fiseha Baraki
Berhe, Birhanu Debesay
Sahle, Tagel Elias
Mossa, Mohammed Mebrahtu
Source
International Journal of Agronomy
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
This study was conducted to develop an efficient regeneration protocol used for sesame genetic transformation.
Published regeneration methods using 6-benzylaminopurine (BAP), indol-3-butyric acid (IBA), and α-naphthaleneacetic acid (NAA) were used in this experiment.
Cotyledon explants of 14 Ethiopian genotypes collected from Humera Agricultural Research Center (HuARC) were used.
Optimized culture conditions were used to investigate the regeneration response of different genotypes.
Significant interactions between hormone treatments, MS media, and genotypes for shoot and root regeneration were recorded.
Results showed that culture of cotyledons were developed and used as a source of explants for shoot regeneration.
The highest shoot number, leaf number, and shoot length were recorded on explants cultured on 1.00 mg·L−1 BAP + 1.00 mg·L−1 NAA in Hirhir and Aberghele, 0.75 mg·L−1 BAP + 1.00 mg·L−1 NAA in Baha Zeyit, and 1.0 mg·L−1 BAP + 1.00 mg·L−1 NAA in Humera 1, respectively.
The lowest shoot number, leaf number, and shoot length were observed on explants cultured on the control in Gondar 1, Borkana, and Baha Necho, Borkana and Beha Necho, and Baha Necho, respectively.
Genotypes with well-developed shoots were transferred to root induction media.
Under rooting media, the best mean, root number, and root length were observed in Aberghele and ACC44 at 0.1 mg·L−1 IBA and 0.2 mg·L−1 NAA, respectively.
Standardizing genotypes trait data to mean 0 and unity variance has helped to group 14 genotypes into four distinct clusters.
Hirhir, Abeghele, Baha Zeyit, and Humera 1 were the best genotypes.
These findings lay ground to Ethiopian sesame to do further genetic transformation aiming at improving the crop.
American Psychological Association (APA)
Weldemichael, Micheale Yifter& Bayratsion, Yemane Tsehaye& Sbhatu, Desta Berhe& Abraha, Girmay Gebresamuel& Juhar, Hagos Mohammedseid& Birhan Kassa, Abraha…[et al.]. 2020. In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in Ethiopia. International Journal of Agronomy،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1167510
Modern Language Association (MLA)
Weldemichael, Micheale Yifter…[et al.]. In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in Ethiopia. International Journal of Agronomy No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1167510
American Medical Association (AMA)
Weldemichael, Micheale Yifter& Bayratsion, Yemane Tsehaye& Sbhatu, Desta Berhe& Abraha, Girmay Gebresamuel& Juhar, Hagos Mohammedseid& Birhan Kassa, Abraha…[et al.]. In Vitro Shoot Regeneration of Oil Seed Crop Sesamum indicum L. from Seedling Cotyledon Explant to Lay Ground for Genetic Transformation in Ethiopia. International Journal of Agronomy. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1167510
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167510