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Molecular Study of Acinetobacter baumannii Isolates for Metallo-β-Lactamases and Extended-Spectrum-β-Lactamases Genes in Intensive Care Unit, Mansoura University Hospital, Egypt
Joint Authors
Alkasaby, Nashwa M.
El Sayed Zaki, Maysaa
Source
International Journal of Microbiology
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-08
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Acinetobacter baumannii (A.
baumannii) has been known as a causative pathogen of hospital acquired infections.
The aim of this study is to examine the presence of A.
baumannii among clinical isolates from intensive care unit (ICU) in Mansoura University Hospital (MUH), its antibiotic resistance pattern, and prevalence of antibiotic resistance genes metallo-β-lactamases (MBLs) and extended-spectrum-β-lactamases (ESBLs) among A.
baumannii isolates.
A.
baumannii was identified by colony morphology, API 20E, and confirmed by detecting the bla OXA-51-like carbapenemase gene by PCR.
Phenotypic expression of MBLs resistance was demonstrated by Combined Disk Test (CDT) in 273 isolates (97.5%) and of ESBLs was demonstrated by double disc synergy method (DDST) in 6 isolates (2.1%).
MBLs genes were positive in 266 isolates (95%) and ESBLs genes were positive in 8 isolates (2.9%).
The most frequent genes of MBLs studied genes were IMP (95.7%) followed by SIM and GIM (47.1% and 42.9%; resp.).
For ESBL genes, the most frequent gene was TEM (2.9%).
From this study, we conclude that multidrug resistant (MDR) A.
baumannii with MBLs activity was the most common isolate.
Careful monitoring for the presence of MDR A.
baumannii among hospitalized patients is recommended to avoid wide dissemination of antibiotic resistance.
American Psychological Association (APA)
Alkasaby, Nashwa M.& El Sayed Zaki, Maysaa. 2017. Molecular Study of Acinetobacter baumannii Isolates for Metallo-β-Lactamases and Extended-Spectrum-β-Lactamases Genes in Intensive Care Unit, Mansoura University Hospital, Egypt. International Journal of Microbiology،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1167558
Modern Language Association (MLA)
Alkasaby, Nashwa M.& El Sayed Zaki, Maysaa. Molecular Study of Acinetobacter baumannii Isolates for Metallo-β-Lactamases and Extended-Spectrum-β-Lactamases Genes in Intensive Care Unit, Mansoura University Hospital, Egypt. International Journal of Microbiology No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1167558
American Medical Association (AMA)
Alkasaby, Nashwa M.& El Sayed Zaki, Maysaa. Molecular Study of Acinetobacter baumannii Isolates for Metallo-β-Lactamases and Extended-Spectrum-β-Lactamases Genes in Intensive Care Unit, Mansoura University Hospital, Egypt. International Journal of Microbiology. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1167558
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167558