A GC-MS-Based Metabolomics Investigation of the Protective Effect of Liu-Wei-Di-Huang-Wan in Type 2 Diabetes Mellitus Mice

Joint Authors

Yu, Rong
Huang, Jian-hua
He, Dan
Chen, Lin
Du, Qing
Cai, Ping
Zhang, Shui-han

Source

International Journal of Analytical Chemistry

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-08-13

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Chemistry
Science

Abstract EN

Aim of the Study.

The present study aimed to establish a metabolic profile and to investigate the changes of endogenous metabolites in diabetic mice treated with LWDHW.

We further investigated the metabolic mechanism of action for the hypoglycemic activity of LWDHW and its possible role in reducing the risk of renal failure in type 2 diabetic mice.

Materials and Methods.

MKR mice were used for the development of diabetes with high-fat diet feeding.

These mice were further injected with streptozocin (STZ) to aggravate kidney failure.

Fasting blood glucose (FBG) and urinary albumin-to-creatinine ratio (ACR values) were determined to validate the successful establishment of diabetic models with desired kidney dysfunction.

Metabolomics approach coupled with gas chromatography-mass spectrometry (GC-MS) and random forest (RF) algorithm was proposed to discover the metabolic differences among model group and control group as well as to examine the therapeutic efficacy of traditional Chinese medicine, Liu-Wei-Di-Huang-Wan (LWDHW), in diabetes and associated kidney failure.

Results.

Some metabolites such as 3-hydroxybutyric acid, citric acid, hexadecanoic acid, and octadecanoic acid showed significant differences between the control group and model group.

Treatment with LWDHW resulted in a significant decrease in FBG and ACR values.

These results suggested that LWDHW could have beneficial effects in diabetes-associated renal failure.

American Psychological Association (APA)

Huang, Jian-hua& He, Dan& Chen, Lin& Du, Qing& Yu, Rong& Cai, Ping…[et al.]. 2020. A GC-MS-Based Metabolomics Investigation of the Protective Effect of Liu-Wei-Di-Huang-Wan in Type 2 Diabetes Mellitus Mice. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167587

Modern Language Association (MLA)

Yu, Rong…[et al.]. A GC-MS-Based Metabolomics Investigation of the Protective Effect of Liu-Wei-Di-Huang-Wan in Type 2 Diabetes Mellitus Mice. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1167587

American Medical Association (AMA)

Huang, Jian-hua& He, Dan& Chen, Lin& Du, Qing& Yu, Rong& Cai, Ping…[et al.]. A GC-MS-Based Metabolomics Investigation of the Protective Effect of Liu-Wei-Di-Huang-Wan in Type 2 Diabetes Mellitus Mice. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167587

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1167587