Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage
Joint Authors
de la Garza-Ramos, Myriam Angelica
Galán-Wong, Luis Jesús
Gamiño-Hernández, Rossana
Fernández-Chapa, David
García-Díaz, Graciela
Guajardo-Barbosa, Claudio
Luna-Olvera, Hugo Alberto
Source
International Journal of Microbiology
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-20
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
This study was carried out to determine the persistence of toxicity of fermentation extracts of Bacillus thuringiensis var.
israelensis after more than three decades of storage.
For this purpose, a population of Aedes aegypti was established.
The mortality rate of 20 spore-crystal extracts purified using the acetone-lactose coprecipitation method was measured and evaluated by bioassays according to a modified WHO protocol.
The extracts with the highest mortality rate were determined in triplicate by their LD50 and LD98.
All extracts showed toxicity at the highest tested dose (1000 ppm) and some, such as strains 3260 and 3501, still killed larvae at doses as low as 0.01 ppm.
These data are surprising because no study on the activity of B.
thuringiensis toxic proteins after such a long storage time has been reported.
American Psychological Association (APA)
Galán-Wong, Luis Jesús& Gamiño-Hernández, Rossana& Fernández-Chapa, David& García-Díaz, Graciela& de la Garza-Ramos, Myriam Angelica& Guajardo-Barbosa, Claudio…[et al.]. 2017. Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage. International Journal of Microbiology،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1167596
Modern Language Association (MLA)
Galán-Wong, Luis Jesús…[et al.]. Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage. International Journal of Microbiology No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1167596
American Medical Association (AMA)
Galán-Wong, Luis Jesús& Gamiño-Hernández, Rossana& Fernández-Chapa, David& García-Díaz, Graciela& de la Garza-Ramos, Myriam Angelica& Guajardo-Barbosa, Claudio…[et al.]. Persistence of Toxic Activity of Fermentation Extracts from Bacillus thuringiensis var. israelensis after More Than Three Decades of Storage. International Journal of Microbiology. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1167596
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167596