Comparative Analysis of Free Amino Acids and Nucleosides in Different Varieties of Mume Fructus Based on Simultaneous Determination and Multivariate Statistical Analyses

Joint Authors

Wang, Rui
Yuan, Qingjun
Li, Xiaoli
Ou, Jinmei
Huang, Luqi
Fang, Chengwu
Wu, Deling

Source

International Journal of Analytical Chemistry

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-08-01

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Chemistry
Science

Abstract EN

Mume Fructus (MF) contains a variety of organic acids, free amino acids, and nucleoside components, and studies have not yet analyzed the relationship between the components of free amino acids and nucleosides with the varieties of MF.

A rapid and sensitive method was established for simultaneous determination of 21 free amino acids and 9 nucleosides in MF by ultrafast liquid chromatography-mass spectrometry.

The analysis was carried out on a Waters XBridge Amide column (100 mm × 2.1 mm, 3.5 μm) with elution by the mobile phase of 0.2% aqueous formic acid (A) and 0.2% formic acid acetonitrile (B) at a flow rate of 0.2 mL/min with 1 μL per injection.

The column temperature was maintained at 30°C.

The target compounds were analyzed by the positive ion multiple reaction monitoring (MRM) mode.

The comprehensive evaluation of the samples was carried out by principal component analysis (PCA) and technique for order preference by similarity to an ideal solution (TOPSIS) analysis.

Results showed the method could simultaneously determine 30 components in MF.

The content of total analytes in six mainstream varieties was different, exhibited the order Nangao > Daqingmei > Zhaoshuimei > Yanmei > Shishengme > Baimei, and aspartic acid and adenosine were the most abundant amino acid and nucleoside.

PCA and OPLS-DA could easily distinguish the samples, and 11 components could be chemical markers of sample classification.

TOPSIS implied that the quality of Nangao and Daqingmei was superior to the other varieties.

The results could provide a reliable basis for quality evaluation and utilisation of medicinal and edible MF.

American Psychological Association (APA)

Ou, Jinmei& Wang, Rui& Li, Xiaoli& Huang, Luqi& Yuan, Qingjun& Fang, Chengwu…[et al.]. 2020. Comparative Analysis of Free Amino Acids and Nucleosides in Different Varieties of Mume Fructus Based on Simultaneous Determination and Multivariate Statistical Analyses. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167651

Modern Language Association (MLA)

Wang, Rui…[et al.]. Comparative Analysis of Free Amino Acids and Nucleosides in Different Varieties of Mume Fructus Based on Simultaneous Determination and Multivariate Statistical Analyses. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1167651

American Medical Association (AMA)

Ou, Jinmei& Wang, Rui& Li, Xiaoli& Huang, Luqi& Yuan, Qingjun& Fang, Chengwu…[et al.]. Comparative Analysis of Free Amino Acids and Nucleosides in Different Varieties of Mume Fructus Based on Simultaneous Determination and Multivariate Statistical Analyses. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167651

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1167651