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Morphological and Genetic Diversity of Rhizobia Nodulating Cowpea (Vigna unguiculata L.) from Agricultural Soils of Lower Eastern Kenya
Joint Authors
Wagacha, John M.
Ondieki, Damaris K.
Nyaboga, Evans N.
Mwaura, Francis B.
Source
International Journal of Microbiology
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Limited nitrogen (N) content in the soil is a major challenge to sustainable and high crop production in many developing countries.
The nitrogen fixing symbiosis of legumes with rhizobia plays an important role in supplying sufficient N for legumes and subsequent nonleguminous crops.
To identify rhizobia strains which are suitable for bioinoculant production, characterization of rhizobia is a prerequisite.
The objective of this study was to assess the morphological and genetic diversity of rhizobia that nodulates cowpea in agricultural soils of lower eastern Kenya.
Twenty-eight rhizobia isolates were recovered from soil samples collected from farmers’ fields in Machakos, Makueni, and Kitui counties in lower eastern Kenya and characterized based on morphological characteristics.
Thirteen representative isolates were selected and characterized using BOX repetitive element PCR fingerprinting.
Based on the dendrogram generated from morphological characteristics, the test isolates were distributed into two major clusters at a similarity of 75%.
Phylogenetic tree, based on BOX repetitive element PCR, grouped the isolates into two clusters at 90% similarity level.
The clustering of the isolates did not show a relationship to the origin of soil samples, although the isolates were genetically diverse.
This study is a prerequisite to the selection of suitable cowpea rhizobia to develop bioinoculants for sustainable crop production in Kenya.
American Psychological Association (APA)
Ondieki, Damaris K.& Nyaboga, Evans N.& Wagacha, John M.& Mwaura, Francis B.. 2017. Morphological and Genetic Diversity of Rhizobia Nodulating Cowpea (Vigna unguiculata L.) from Agricultural Soils of Lower Eastern Kenya. International Journal of Microbiology،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1167657
Modern Language Association (MLA)
Ondieki, Damaris K.…[et al.]. Morphological and Genetic Diversity of Rhizobia Nodulating Cowpea (Vigna unguiculata L.) from Agricultural Soils of Lower Eastern Kenya. International Journal of Microbiology No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1167657
American Medical Association (AMA)
Ondieki, Damaris K.& Nyaboga, Evans N.& Wagacha, John M.& Mwaura, Francis B.. Morphological and Genetic Diversity of Rhizobia Nodulating Cowpea (Vigna unguiculata L.) from Agricultural Soils of Lower Eastern Kenya. International Journal of Microbiology. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1167657
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167657