Establishment and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Tigecycline in Critically Ill Patients

Joint Authors

Wang, Yifan
Yao, Fen
Wu, Zheng
Wang, Xipei
Hou, Yating
Lan, Jinhua
Wang, Yirong
Chen, Chunbo

Source

International Journal of Analytical Chemistry

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-12-29

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Chemistry
Science

Abstract EN

Utilizing tigecycline-d9 as an internal standard (IS), we establish and validate a simple, effective, and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantitative measurement of tigecycline (TGC) in patient plasma.

Acetonitrile was used as a precipitant to process plasma samples by a protein precipitation method.

The analyte and IS were separated on an HSS T3 (2.1 × 100 mm, 3.5 μm) chromatographic column using isocratic program with a mobile phase comprising of 80% solvent A (water containing 0.1% formic acid (v/v) with 5 mM ammonium acetate) and 20% solvent B (acetonitrile) with a flow rate of 0.3 mL/min.

The mass spectrometer, scanning in multireaction monitoring (MRM) mode and using an electrospray ion source (ESI), operated in the positive-ion mode.

The ion pairs used for quantitative analysis were m/z 586.4 ⟶ 513.3 and m/z 595.5 ⟶ 514.3 for TGC and the IS, respectively.

The range of the linear calibration curve obtained with this approach was 50–5000 ng/ml.

Intra- and interbatch precision for TGC quantitation were less than 7.2%, and the accuracy ranged from 93.4 to 101.8%.

The IS-normalized matrix effect was 87 to 104%.

Due to its high precision and accuracy, this novel method allows for fast quantitation of TGC with a total analysis time of 2 min.

This approach was effectively applied to study the pharmacokinetics of TGC in critically ill adult patients.

American Psychological Association (APA)

Yao, Fen& Wang, Yifan& Hou, Yating& Wang, Xipei& Lan, Jinhua& Wu, Zheng…[et al.]. 2020. Establishment and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Tigecycline in Critically Ill Patients. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167684

Modern Language Association (MLA)

Wang, Yifan…[et al.]. Establishment and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Tigecycline in Critically Ill Patients. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1167684

American Medical Association (AMA)

Yao, Fen& Wang, Yifan& Hou, Yating& Wang, Xipei& Lan, Jinhua& Wu, Zheng…[et al.]. Establishment and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Tigecycline in Critically Ill Patients. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167684

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1167684