Pharmacokinetics of Lusutrombopag, a Novel Thrombopoietin Receptor Agonist, in Rats by UPLC-MSMS

Joint Authors

Zhou, Quan
Chen, Feifei
Zhou, Yunfang
Meng, Deru
Geng, Peiwu
Hua, Ailian
Ji, Weiping
Wang, Changxiong
Wang, Shuanghu
Hu, Liming
Wang, Bo

Source

International Journal of Analytical Chemistry

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-05-30

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Chemistry
Science

Abstract EN

Lusutrombopag is a second oral thrombopoietin (TPO) receptor agonist that selectively acts on human TPO receptors.

In the study, UPLC-MS/MS was used to establish a selective and sensitive method to determine lusutrombopag with poziotinib as IS (internal standard) in rat plasma.

Samples were prepared by precipitating protein with acetonitrile as a precipitant.

Separation of lusutrombopag and poziotinib was performed on a CORTECS UPLC C18 column (2.1 ∗ 50 mm, 1.6 μm).

The mobile phase (acetonitrile and water containing 0.1% formic acid) with gradient elution was set at a flow rate of 0.4 ml/min.

The mass spectrometric measurement was conducted under positive ion mode using multiple reaction monitoring (MRM) of m/z 592.97 ⟶ 491.02 for lusutrombopag and m/z for poziotinib (IS) 492.06 ⟶ 354.55.

The linear calibration curve of the concentration range was 2–2000 ng/ml for lusutrombopag, with a lower limit of quantification (LLOQ) of 2 ng/ml.

RSD of interday and intraday precision were both no more than 9.66% with the accuracy ranging from 105.82% to 108.27%.

The extraction recovery of lusutrombopag was between 82.15% and 90.34%.

The developed and validated method was perfectly used in the pharmacokinetic study of lusutrombopag after oral administration in rats.

American Psychological Association (APA)

Wang, Bo& Chen, Feifei& Zhou, Quan& Zhou, Yunfang& Meng, Deru& Geng, Peiwu…[et al.]. 2020. Pharmacokinetics of Lusutrombopag, a Novel Thrombopoietin Receptor Agonist, in Rats by UPLC-MSMS. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1167688

Modern Language Association (MLA)

Wang, Bo…[et al.]. Pharmacokinetics of Lusutrombopag, a Novel Thrombopoietin Receptor Agonist, in Rats by UPLC-MSMS. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1167688

American Medical Association (AMA)

Wang, Bo& Chen, Feifei& Zhou, Quan& Zhou, Yunfang& Meng, Deru& Geng, Peiwu…[et al.]. Pharmacokinetics of Lusutrombopag, a Novel Thrombopoietin Receptor Agonist, in Rats by UPLC-MSMS. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1167688

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1167688