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Immobilization of Dextranase Using Anionic Natural Polymer Alginate as a Matrix for the Degradation of a Long-Chain Biopolymer (Dextran)
Joint Authors
Shahid, Faiza
Aman, Afsheen
Qader, Shah Ali Ul
Source
International Journal of Polymer Science
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Alginate is an inexpensive, nontoxic, valuable biopolymer utilized in the study for the immobilization of commercially applicable biocatalyst dextranase.
Dextranase was immobilized by an entrapment method, and alginate hydrogel spheres were synthesized after optimizing several parameters.
A sodium alginate concentration of 4.0% was noticed to be suitable along with a calcium chloride concentration of 0.2 molar after providing a curing time of 20 minutes.
After comparing the characteristics of the entrapped enzyme with those of the soluble one, it was observed that the characteristics were more or less the same except for the change in reaction time which was noticed to be prolonged in the case of entrapped dextranase while the change in temperature and pH optima was not observed.
The variation in Vmax and Km values of dextranase after entrapment was also noted.
However, after extensive stability examination studies, it was found that dextranase became more stable after entrapment; as a result, it retained more than 50% of its original activity at elevated temperature even after exposure for about 2.0 hours.
The reusability of dextranase was up to 7.0 cycles after performing catalytic activity under constant condition.
American Psychological Association (APA)
Shahid, Faiza& Aman, Afsheen& Qader, Shah Ali Ul. 2019. Immobilization of Dextranase Using Anionic Natural Polymer Alginate as a Matrix for the Degradation of a Long-Chain Biopolymer (Dextran). International Journal of Polymer Science،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1167733
Modern Language Association (MLA)
Shahid, Faiza…[et al.]. Immobilization of Dextranase Using Anionic Natural Polymer Alginate as a Matrix for the Degradation of a Long-Chain Biopolymer (Dextran). International Journal of Polymer Science No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1167733
American Medical Association (AMA)
Shahid, Faiza& Aman, Afsheen& Qader, Shah Ali Ul. Immobilization of Dextranase Using Anionic Natural Polymer Alginate as a Matrix for the Degradation of a Long-Chain Biopolymer (Dextran). International Journal of Polymer Science. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1167733
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167733