Aptamer-Based Colorimetric Probe for trans-Zeatin Detection Using Unmodified Gold Nanoparticle
Joint Authors
Zhang, Xiwei
Sun, Ping
Wang, Xianxiang
Source
International Journal of Analytical Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Trans-Zeatin is the major active phytohormone in immature corn kernels.
Herein, a highly sensitive, good selective and simple aptamer-based colorimetric method for the detection of trans-zeatin was constructed.
The selected aptamer sequence binds with trans-zeatin and induces a duplex-to-aptamer structure switching.
The gold nanoparticles (AuNPs) solution is stable with high-concentration salt, which is protected by red complementary DNA.
In the absence of trans-zeatin, the color of AuNPs changed from red to blue because aptamer DNA and complementary DNA form double-stranded DNA.
Thus, the ratio of absorbance intensities (A522/A650) of AuNPs is changed with the concentration of trans-zeatin.
The color change could be observed by the naked eye.
The linear range of this method covers a large variation of trans-zeatin concentration from 0.05 to 0.75 μM.
The detection limit is 0.037 μM.
Moreover, this method was applied successfully to detect trans-zeatin in real plant samples.
American Psychological Association (APA)
Sun, Ping& Zhang, Xiwei& Wang, Xianxiang. 2020. Aptamer-Based Colorimetric Probe for trans-Zeatin Detection Using Unmodified Gold Nanoparticle. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167766
Modern Language Association (MLA)
Sun, Ping…[et al.]. Aptamer-Based Colorimetric Probe for trans-Zeatin Detection Using Unmodified Gold Nanoparticle. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1167766
American Medical Association (AMA)
Sun, Ping& Zhang, Xiwei& Wang, Xianxiang. Aptamer-Based Colorimetric Probe for trans-Zeatin Detection Using Unmodified Gold Nanoparticle. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167766
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167766