A Process Engineering Approach to Improve Production of P(3HB) by Cupriavidus necator from Used Cooking Oil
Joint Authors
Reis, Maria A. M.
Cruz, Madalena V.
Gouveia, Ana Rosa
Dionísio, Madalena
Freitas, Filomena
Source
International Journal of Polymer Science
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Different feeding strategies, namely, exponential feeding and DO-stat mode, were implemented for the production of poly(3-hydroxybutyrate), P(3HB), by Cupriavidus necator DSM 428 with used cooking oil (UCO) as the sole carbon source.
With the exponential feeding strategy, a cell dry mass of 21.3 ± 0.9 g L−1 was obtained, with a polymer content of 84.0 ± 4.5 wt.%, giving an overall volumetric productivity of 4.5 ± 0.2 g L−1 day−1.
However, the highest P(3HB) volumetric productivity, 12.6 ± 0.8 g L−1 day−1, was obtained when the DO-stat mode was implemented together with the use of ammonium hydroxide for pH control, which served as an additional nitrogen source and allowed to reach higher cell dry mass (7.8 ± 0.6 g L−1).
The P(3HB) obtained in all experiments had a high molecular mass, ranging from 0.6 × 105 to 2.6 × 105 g mol−1, with low polydispersity indexes of 1.2-1.6.
Melting and glass transition temperatures were also similar for the polymer produced with both cultivation strategy, 174°C and 3.0-4.0°C, respectively.
The polymer exhibited a crystallinity ranging from 52 to 65%.
The DO-stat strategy to feed oil containing substrates as the sole carbon sources was reported for the first time in this study, and the preliminary results obtained show that it is a promising strategy to improve P(3HB) production.
Nevertheless, the process requires further optimization in order to make it economically viable.
American Psychological Association (APA)
Cruz, Madalena V.& Gouveia, Ana Rosa& Dionísio, Madalena& Freitas, Filomena& Reis, Maria A. M.. 2019. A Process Engineering Approach to Improve Production of P(3HB) by Cupriavidus necator from Used Cooking Oil. International Journal of Polymer Science،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1167789
Modern Language Association (MLA)
Cruz, Madalena V.…[et al.]. A Process Engineering Approach to Improve Production of P(3HB) by Cupriavidus necator from Used Cooking Oil. International Journal of Polymer Science No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1167789
American Medical Association (AMA)
Cruz, Madalena V.& Gouveia, Ana Rosa& Dionísio, Madalena& Freitas, Filomena& Reis, Maria A. M.. A Process Engineering Approach to Improve Production of P(3HB) by Cupriavidus necator from Used Cooking Oil. International Journal of Polymer Science. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1167789
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167789