Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA
Joint Authors
García-Alegría, Alejandro Monserrat
Anduro-Corona, Iván
Pérez-Martínez, Cinthia Jhovanna
Guadalupe Corella-Madueño, María Alba
Rascón-Durán, María Lucila
Astiazaran-Garcia, Humberto
Source
International Journal of Analytical Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA.
Microvolumes were used to analyse DNA samples.
Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision value was ≤2% CV.
Trueness based on bias and the percentage of recovery showed bias values lower than Z-test with a 95% confidence level and a recovery percentage within the range (% Rec = 100% ± 5%), and the stability of the samples was 60 days (2–4°C).
American Psychological Association (APA)
García-Alegría, Alejandro Monserrat& Anduro-Corona, Iván& Pérez-Martínez, Cinthia Jhovanna& Guadalupe Corella-Madueño, María Alba& Rascón-Durán, María Lucila& Astiazaran-Garcia, Humberto. 2020. Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167810
Modern Language Association (MLA)
García-Alegría, Alejandro Monserrat…[et al.]. Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1167810
American Medical Association (AMA)
García-Alegría, Alejandro Monserrat& Anduro-Corona, Iván& Pérez-Martínez, Cinthia Jhovanna& Guadalupe Corella-Madueño, María Alba& Rascón-Durán, María Lucila& Astiazaran-Garcia, Humberto. Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167810
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167810