Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector
Joint Authors
Qin, Lin
He, Yuqi
Tan, Daopeng
Yang, Zhou
Zhang, Qianru
Ling, Hua
Du, Yimei
Lu, Yanliu
Xie, Tianpei
Zhou, Xumei
Source
International Journal of Analytical Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A high-performance liquid chromatography method was developed for simultaneous quantification of 18 polyphenolic compounds from the leaves of Blumea balsamifera, including 17 flavonoids and 1 phenylethanone.
The B.
balsamifera extraction was separated by a Kromasil C18 column (250 × 4.6 mm, 5 μm) with a binary gradient mobile phase consisting of acetonitrile and 0.2% aqueous acetic acid.
A photodiode array detector (PDA) was used to record the signals of investigated constituents.
The linearity, sensitivity, stability, precision, and accuracy of the established assay methods were assessed to meet the requirements of quantitative determination.
Samples extracted by reflux in 25 mL of 80% methanol for 30 minutes were selected for the extraction method.
The 18 compounds were accurately identified by comparing with the reference compounds.
The purity of each peak was confirmed by the base peak in the mass spectrum.
The contents of 18 compounds in Blumea samples from four different regions were successfully determined.
The results also showed that 3,3′,5,7-tetrahydroxy-4′-methoxyflavanone was the most abundant constituent, which could be used as a potential chemical marker for quality control of B.
balsamifera and Chinese patent medications containing B.
balsamifera herb.
American Psychological Association (APA)
Tan, Daopeng& Yang, Zhou& Zhang, Qianru& Ling, Hua& Du, Yimei& Lu, Yanliu…[et al.]. 2020. Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167827
Modern Language Association (MLA)
Tan, Daopeng…[et al.]. Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1167827
American Medical Association (AMA)
Tan, Daopeng& Yang, Zhou& Zhang, Qianru& Ling, Hua& Du, Yimei& Lu, Yanliu…[et al.]. Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167827
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167827