Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung)‎ by High-Performance Liquid Chromatography with Photodiode Array Detector

Joint Authors

Qin, Lin
He, Yuqi
Tan, Daopeng
Yang, Zhou
Zhang, Qianru
Ling, Hua
Du, Yimei
Lu, Yanliu
Xie, Tianpei
Zhou, Xumei

Source

International Journal of Analytical Chemistry

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-03-18

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Chemistry
Science

Abstract EN

A high-performance liquid chromatography method was developed for simultaneous quantification of 18 polyphenolic compounds from the leaves of Blumea balsamifera, including 17 flavonoids and 1 phenylethanone.

The B.

balsamifera extraction was separated by a Kromasil C18 column (250 × 4.6 mm, 5 μm) with a binary gradient mobile phase consisting of acetonitrile and 0.2% aqueous acetic acid.

A photodiode array detector (PDA) was used to record the signals of investigated constituents.

The linearity, sensitivity, stability, precision, and accuracy of the established assay methods were assessed to meet the requirements of quantitative determination.

Samples extracted by reflux in 25 mL of 80% methanol for 30 minutes were selected for the extraction method.

The 18 compounds were accurately identified by comparing with the reference compounds.

The purity of each peak was confirmed by the base peak in the mass spectrum.

The contents of 18 compounds in Blumea samples from four different regions were successfully determined.

The results also showed that 3,3′,5,7-tetrahydroxy-4′-methoxyflavanone was the most abundant constituent, which could be used as a potential chemical marker for quality control of B.

balsamifera and Chinese patent medications containing B.

balsamifera herb.

American Psychological Association (APA)

Tan, Daopeng& Yang, Zhou& Zhang, Qianru& Ling, Hua& Qin, Lin& He, Yuqi…[et al.]. 2020. Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167827

Modern Language Association (MLA)

Qin, Lin…[et al.]. Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1167827

American Medical Association (AMA)

Tan, Daopeng& Yang, Zhou& Zhang, Qianru& Ling, Hua& Qin, Lin& He, Yuqi…[et al.]. Simultaneous Quantitative Determination of Polyphenolic Compounds in Blumea balsamifera (Ai-Na-Xiang, Sembung) by High-Performance Liquid Chromatography with Photodiode Array Detector. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1167827

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1167827