Effect of Sulfated Polysaccharide from Undaria pinnatifida (SPUP) on Proliferation, Migration, and Apoptosis of Human Prostatic Cancer
Joint Authors
Xu, Xiaolin
Zhu, Xin
Lu, Wenglong
He, Yandong
Wang, Yihan
Liu, Feng
Source
International Journal of Polymer Science
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
To observe the effect of sulfated polysaccharide from Undaria pinnatifida (SPUP) on proliferation, migration, and apoptosis of human prostatic cancer.
Methods.
DU145 human prostate cancer cells were cultured in vitro, and the proliferation activity both in the control group and the SPUP treatment groups (25, 50, 100, 200 μg/ml) was measured by CCK-8 assay.
The wound healing assay was conducted to detect the cell migration.
Cell apoptosis was measured by flow cytometry.
The protein and mRNA expressions of matrix metalloproteinase-9 (MMP-9) and apoptosis-related factor Bax were detected by qRT-PCR and Western blot.
The expressions of cleaved caspase-3 and cleaved caspase-9 were also determined by Western blot.
Results.
(1) CCK-8 results showed that the proliferative activity of DU145 cells was significantly decreased with the increase of SPUP treatment concentration (P<0.05) in a dose-dependent manner and that the inhibitory effect of SPUP was most significant at 72 h (P<0.05) as compared with the control group; (2) the migration rate of SPUP-treated cells was significantly decreased (P<0.05) as compared with the control group.
And the results of qRT-PCR and Western blot assays showed that SPUP inhibited the expression of MMP-9 in DU145 cells; (3) compared with the control group, the SPUP-treated groups had increased apoptosis of the cells.
The expressions of apoptosis-related factors cleaved caspase-3, cleaved caspase-9, and Bax were upregulated (P<0.05), and the mRNA expression of Bax was increased (P<0.05).
Conclusion.
SPUP showed an antitumor activity in prostatic cancer, and the underlying mechanism may be pertaining to inhibition of migration, proliferation, and induction of apoptosis of cancer cells.
American Psychological Association (APA)
Xu, Xiaolin& Zhu, Xin& Lu, Wenglong& He, Yandong& Wang, Yihan& Liu, Feng. 2019. Effect of Sulfated Polysaccharide from Undaria pinnatifida (SPUP) on Proliferation, Migration, and Apoptosis of Human Prostatic Cancer. International Journal of Polymer Science،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1168233
Modern Language Association (MLA)
Xu, Xiaolin…[et al.]. Effect of Sulfated Polysaccharide from Undaria pinnatifida (SPUP) on Proliferation, Migration, and Apoptosis of Human Prostatic Cancer. International Journal of Polymer Science No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1168233
American Medical Association (AMA)
Xu, Xiaolin& Zhu, Xin& Lu, Wenglong& He, Yandong& Wang, Yihan& Liu, Feng. Effect of Sulfated Polysaccharide from Undaria pinnatifida (SPUP) on Proliferation, Migration, and Apoptosis of Human Prostatic Cancer. International Journal of Polymer Science. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1168233
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1168233