Cellulase-Assisted Extraction, Characterization, and Bioactivity against Rheumatoid Arthritis of Astragalus Polysaccharides
Joint Authors
Cao, Ling
Yu, Mi
Wang, Chonghui
Bao, Yunhui
Zhang, Minghui
He, Ping
Zhang, Yan
Yang, Tingting
Li, Lulu
Li, Gang
Gong, Yun
Source
International Journal of Polymer Science
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This study investigated the effect of cellulase on the isolation of crude Astragalus polysaccharide (APS), analyzed the monosaccharide component of deproteinized APS, detected the molecular weights of purified APS, and examined the biological activities and the preliminary mechanism against rheumatoid arthritis (RA).
Compared with water extraction method, cellulase-assisted extraction increased the yield of crude APS to 154% and polysaccharide contents to 121%.
Crude APS was then purified by ethanol precipitation, Sevag deproteinization, and high-performance liquid chromatography (HPLC) analysis; monosaccharide contents of APS were different after cellulase-assisted method, especially galacturonic acid content which significantly increased.
DEAE-52 cellulose column chromatography isolated three polysaccharide fractions, including a neutral polysaccharide (APS-water) and two acidic polysaccharides (APS-NaCl1 and APS-NaCl2).
Using high-performance gel permeation chromatography (HPGPC), the molecular weights of APS-water, APS-NaCl1, and APS-NaCl2 were identified as 67.7 kDa, 234.1 kDa, and 189.4 kDa, respectively.
Then their therapeutic effects and possible mechanism against RA were explored using type II collagen-induced arthritis (CIA) rat model.
APS could significantly reduce paw swelling, serum concentration of IL-1β and TNF-α, and the expression levels of NF-κB-p65 and IκBα in synovial membranes in CIA rats.
Our study indicated that cellulase significantly increases the yield and polysaccharide contents of crude APS, improves the product quality, and preserves the biological features against RA in CIA rats.
American Psychological Association (APA)
Cao, Ling& Yu, Mi& Wang, Chonghui& Bao, Yunhui& Zhang, Minghui& He, Ping…[et al.]. 2019. Cellulase-Assisted Extraction, Characterization, and Bioactivity against Rheumatoid Arthritis of Astragalus Polysaccharides. International Journal of Polymer Science،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1168310
Modern Language Association (MLA)
Cao, Ling…[et al.]. Cellulase-Assisted Extraction, Characterization, and Bioactivity against Rheumatoid Arthritis of Astragalus Polysaccharides. International Journal of Polymer Science No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1168310
American Medical Association (AMA)
Cao, Ling& Yu, Mi& Wang, Chonghui& Bao, Yunhui& Zhang, Minghui& He, Ping…[et al.]. Cellulase-Assisted Extraction, Characterization, and Bioactivity against Rheumatoid Arthritis of Astragalus Polysaccharides. International Journal of Polymer Science. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1168310
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1168310