Simultaneous Analysis of Twelve Bile Acids by UPLC-MS and Exploration of the Processing Mechanism of Bile Arisaema by Fermentation
Joint Authors
Zhao, Qimiao
Shan, Guoshun
Xu, Dan
Gao, Hui
Shi, Ji
Ju, Chengguo
Lin, Guimei
Zhang, Fan
Jia, Tianzhu
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-09
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Ultrahigh-performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight tandem mass spectrometry (Q/TOF-MS) in the MS/MS mode and UPLC coupled with triple quadrupole mass spectrometry (QqQ-MS) using the multiple reaction monitoring (MRM) mode were used to make a qualitative and quantitative analysis of twelve bile acids in Bile Arisaema.
The fragmentation pathway of twelve bile acids was proposed.
The quantification method showed a good linearity over a wide concentration range (R2 > 0.99), repeatability (RSD < 4.12%), stability (RSD < 4.25%), precision (RSD < 4.06%), and recovery (95.36–102.15%).
Content of twelve compounds in Bile Arisaema varied significantly depending on region.
Chemometric methods, hierarchical clustering analysis (HCA), and principal components analysis (PCA) were successfully used to optimize the fermentation time of the Bile Arisaema.
The results suggested that the Bile Arisaema could complete fermentation in 15 days.
The possible processing mechanism of Bile Arisaema promoted the transformation of conjugated bile acids into free bile acids in fermentation.
American Psychological Association (APA)
Zhao, Qimiao& Shan, Guoshun& Xu, Dan& Gao, Hui& Shi, Ji& Ju, Chengguo…[et al.]. 2019. Simultaneous Analysis of Twelve Bile Acids by UPLC-MS and Exploration of the Processing Mechanism of Bile Arisaema by Fermentation. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1169100
Modern Language Association (MLA)
Zhao, Qimiao…[et al.]. Simultaneous Analysis of Twelve Bile Acids by UPLC-MS and Exploration of the Processing Mechanism of Bile Arisaema by Fermentation. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1169100
American Medical Association (AMA)
Zhao, Qimiao& Shan, Guoshun& Xu, Dan& Gao, Hui& Shi, Ji& Ju, Chengguo…[et al.]. Simultaneous Analysis of Twelve Bile Acids by UPLC-MS and Exploration of the Processing Mechanism of Bile Arisaema by Fermentation. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1169100
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169100