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Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR
Joint Authors
Zhang, Tao
Liu, Cong
Zhou, Wuping
Jiang, Keming
Yin, Chenyu
Liu, Cong
Zhang, Zhiqiang
Li, Haiwen
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-02
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
In this study, an ultrasensitive detection method for aqueous Pb2+ based on digital polymerase chain reaction (dPCR) technology and a Pb2+-dependent DNAzyme was developed.
In the presence of Pb2+, the Gr-5 DNAzyme was activated and catalyzed the hydrolytic cleavage of the substrate strand, resulting in an increase in the amount of template DNA available for dPCR and a resultant change in the number of droplets showing a positive signal.
Moreover, the detection system was found to be sensitive and stable in environmental sample detection.
In summary, an ultrasensitive quantitative detection method for Pb2+ within environmental substrates was established.
American Psychological Association (APA)
Zhang, Tao& Liu, Cong& Zhou, Wuping& Jiang, Keming& Yin, Chenyu& Liu, Cong…[et al.]. 2019. Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1169122
Modern Language Association (MLA)
Zhang, Tao…[et al.]. Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1169122
American Medical Association (AMA)
Zhang, Tao& Liu, Cong& Zhou, Wuping& Jiang, Keming& Yin, Chenyu& Liu, Cong…[et al.]. Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1169122
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169122