A Sensitive Fluorescence Biosensor for Silver Ions (Ag+) Detection Based on C-Ag+-C Structure and Exonuclease III-Assisted Dual-Recycling Amplification
Joint Authors
Li, Yubin
Yuan, Jiaming
Xu, Zexi
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A C-Ag+-C structure-based fluorescence biosensor with novel combination design of exonuclease III (Exo III) dual-recycling amplification is proposed for the application of silver ions (Ag+) detection.
Since oligo-1 involves C-C mismatches, the presence of Ag+ can be captured to form C-Ag+-C base pairs, which results in a double-helix structure with a blunt terminus.
The double-helix structure can be cleaved by EXO III to release short mononucleotide fragments (trigger DNA) and Ag+.
Released Ag+ can form new bindings with oligo-1, and other trigger DNA can be produced in the digestion cycles.
Hybridization with the signal DNA (oligo-2) transforms a trigger DNA into double-stranded DNA with blunt terminus which can be cleaved by Exo III to reproduce the trigger DNA and form guanine- (G-) quadruplex DNA.
The trigger DNA returns free to the solution and hybridizes with another signal DNA, which realizes the dual-recycling amplification.
The G-quadruplex DNA can be reported by N-methylmesoporphyrin IX (NMM), a specific G-quadruplex DNA fluorochrome.
This method allows Ag+ to be determined in the 5 to 1500 pmol/L concentration range, with a 2 pmol/L detection limit, and it has been successfully applied to the detection of Ag+ in real samples.
American Psychological Association (APA)
Li, Yubin& Yuan, Jiaming& Xu, Zexi. 2019. A Sensitive Fluorescence Biosensor for Silver Ions (Ag+) Detection Based on C-Ag+-C Structure and Exonuclease III-Assisted Dual-Recycling Amplification. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1169131
Modern Language Association (MLA)
Li, Yubin…[et al.]. A Sensitive Fluorescence Biosensor for Silver Ions (Ag+) Detection Based on C-Ag+-C Structure and Exonuclease III-Assisted Dual-Recycling Amplification. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1169131
American Medical Association (AMA)
Li, Yubin& Yuan, Jiaming& Xu, Zexi. A Sensitive Fluorescence Biosensor for Silver Ions (Ag+) Detection Based on C-Ag+-C Structure and Exonuclease III-Assisted Dual-Recycling Amplification. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1169131
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169131